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验证数据展示

  • WB analysis of Jurkat using 26859-1-AP

    WB analysis of Jurkat using 26859-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26859-1-AP (RFX1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 26859-1-AP

    IP experiment of Jurkat using 26859-1-AP

    IP result of anti-RFX1 (IP:26859-1-AP, 4ug; Detection:26859-1-AP 1:500) with Jurkat cells lysate 2030 ug.

  • Featured Product
  • KD/KO验证

RFX1 Polyclonal antibody

RFX1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 26859-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IP, ELISA and More (2)

别名

EF C, Enhancer factor C, Regulatory factor X 1, RFX, RFX1, Transcription factor RFX1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HL-60 cells
Positive IP detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26859-1-AP targets RFX1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原

CatNo: Ag25442

Product name: Recombinant human RFX1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 62-289 aa of BC049826

Sequence: QPQAQPPGGQKQYVTELPAVPAPSQPTGAPTPSPAPQQYIVVTVSEGAMRASETVSEASPGSTASQTGVPTQVVQQVQGTQQRLLVQTSVQAKPGHVSPLQLTNIQVPQQALPTQRLVVQSAAPGSKGGQVSLTVHGTQQVHSPPEQSPVQANSSSSKTAGAPTGTVPQQLQVHGVQQSVPVTQERSVVQATPQAPKPGPVQPLTVQGLQPVHVAQEVQQLQQVPVPH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regulatory factor X, 1 (influences HLA class II expression)
别名 EF C, Enhancer factor C, Regulatory factor X 1, RFX, RFX1, Transcription factor RFX1
计算分子量 105 kDa
观测分子量 135 kDa
GenBank蛋白编号BC049826
基因名称 RFX1
Gene ID (NCBI) 5989
RRIDAB_2880659
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22670
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RFX1, also named as MHC class II regulatory factor RFX1, is a 979 amino acid protein which contains 1 RFX-type winged-helix DNA-binding domain and belongs to the RFX family. RFX1 can binds DNA as a homodimer and heterodimerizes with RFX2 and RFX3. RFX1 as a regulatory factor is essential for MHC class II genes expression. RFX1 protein is modified after translation and its molecular weight could be migrate to 130-140 kDa (PMID: 20189986 ).

实验方案

Product Specific Protocols
WB protocol for RFX1 antibody 26859-1-APDownload protocol
IP protocol for RFX1 antibody 26859-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanIF,WB

Int J Biol Sci

Hypoxia-induced exosomes facilitate lung pre-metastatic niche formation in hepatocellular carcinoma through the miR-4508-RFX1-IL17A-p38 MAPK-NF-κB pathway

Authors - Wentao Jia
  • KD Validated
View Article
human,mouseIHC

Ren Fail

Proteomics reveals the key transcription-related factors mediating obstructive nephropathy in pediatric patients and mice

Authors - Hualin Cao
View Article