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验证数据展示

  • WB analysis of Jurkat using 26859-1-AP

    WB analysis of Jurkat using 26859-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26859-1-AP (RFX1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 26859-1-AP

    IP experiment of Jurkat using 26859-1-AP

    IP result of anti-RFX1 (IP:26859-1-AP, 4ug; Detection:26859-1-AP 1:500) with Jurkat cells lysate 2030 ug.

  • Featured Product
  • KD/KO验证

RFX1 Polyclonal antibody

RFX1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 26859-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IP, ELISA and More (2)

别名

EF C, Enhancer factor C, Regulatory factor X 1, RFX, RFX1, Transcription factor RFX1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HL-60 cells
Positive IP detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26859-1-AP targets RFX1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 RFX1 fusion protein Ag25442 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regulatory factor X, 1 (influences HLA class II expression)
别名 EF C, Enhancer factor C, Regulatory factor X 1, RFX, RFX1, Transcription factor RFX1
计算分子量 105 kDa
观测分子量 135 kDa
GenBank蛋白编号BC049826
基因名称 RFX1
Gene ID (NCBI) 5989
RRIDAB_2880659
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22670
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RFX1, also named as MHC class II regulatory factor RFX1, is a 979 amino acid protein which contains 1 RFX-type winged-helix DNA-binding domain and belongs to the RFX family. RFX1 can binds DNA as a homodimer and heterodimerizes with RFX2 and RFX3. RFX1 as a regulatory factor is essential for MHC class II genes expression. RFX1 protein is modified after translation and its molecular weight could be migrate to 130-140 kDa (PMID: 20189986 ).

实验方案

Product Specific Protocols
WB protocol for RFX1 antibody 26859-1-APDownload protocol
IP protocol for RFX1 antibody 26859-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanIF,WB

Int J Biol Sci

Hypoxia-induced exosomes facilitate lung pre-metastatic niche formation in hepatocellular carcinoma through the miR-4508-RFX1-IL17A-p38 MAPK-NF-κB pathway

Authors - Wentao Jia
  • KD Validated
View Article
human,mouseIHC

Ren Fail

Proteomics reveals the key transcription-related factors mediating obstructive nephropathy in pediatric patients and mice

Authors - Hualin Cao
View Article