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验证数据展示

  • WB analysis of mouse lung using 19893-1-AP (same clone as 19893-1-PBS)

    WB analysis of mouse lung using 19893-1-AP (same clone as 19893-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 19893-1-AP (RFWD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 19893-1-AP (same clone as 19893-1-PBS)

    IHC staining of human testis using 19893-1-AP (same clone as 19893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 19893-1-AP (RFWD3 antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 19893-1-AP (same clone as 19893-1-PBS)

    IHC staining of human testis using 19893-1-AP (same clone as 19893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 19893-1-AP (RFWD3 antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 19893-1-AP (same clone as 19893-1-PBS)

    IF Staining of HEK-293 using 19893-1-AP (same clone as 19893-1-PBS)

    Immunofluorescent analysis of 293 cells, using RFWD3 antibody 19893-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RFWD3 Polyclonal antibody, PBS Only

RFWD3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 19893-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

E3 ubiquitin-protein ligase RFWD3, EC:2.3.2.27, RING finger and WD repeat domain-containing protein 3, RING finger protein 201, RNF201

缓冲液配方: 
偶联物:  Unconjugated
规格价格库存

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产品信息

19893-1-PBS targets RFWD3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13728

Product name: Recombinant human RFWD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 427-774 aa of BC002574

Sequence: SQGQHKHKYHFQKTFTVSQAGNCRIMAYCDALSCLVISQPSPQASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGSILVYDVRNTSSHVQELVAQKARCPLVSLSYMPRAASAAFPYGGVLAGTLEDASFWEQKMDFSHWPHVLPLEPGGCIDFQTENSSRHCLVTYRPDKNHTTIRSVLMEMSYRLDDTGNPICSCQPVHTFFGGPTCKLLTKNAIFQSPENDGNILVCTGDEAANSALLWDAASGSLLQDLQTDQPVLDICPFEVNRNSYLATLTEKMVHIYKWE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger and WD repeat domain 3
别名 E3 ubiquitin-protein ligase RFWD3, EC:2.3.2.27, RING finger and WD repeat domain-containing protein 3, RING finger protein 201, RNF201
计算分子量 774 aa, 85 kDa
观测分子量 85 kDa
GenBank蛋白编号BC002574
基因名称 RFWD3
Gene ID (NCBI) 55159
RRIDAB_10639521
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6PCD5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RFWD3, also named as RNF201, is a E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. RFWD3 may act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, It interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.(PMID: 20173098 ) From BLAST result, this antibody is specific to RFWD3. 45kd and 55kd maybe splicesome of RFWD3.

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