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验证数据展示

  • WB analysis using 17854-1-AP

    WB analysis using 17854-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 17854-1-AP (RFTN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 17854-1-AP

    IHC staining of human liver cancer using 17854-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17854-1-AP (RFTN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RFTN2 Polyclonal antibody

RFTN2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17854-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

C2orf11, Raft linking protein 2, Raftlin 2, raftlin family member 2, RFTN2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue, mouse brain tissue, mouse testis tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17854-1-AP targets RFTN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag12307

Product name: Recombinant human RFTN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 3-501 aa of BC111069

Sequence: CGLRKLEDPDDSSPGKIFSTLKRPQVETKTEFAYEYVLLDFTLQASSNPEVIKINSILDIVTKVENYYLKGYIVGAIHPVIQPVGQRKHLPASYLYRVVLLRLKLSPKNSAAPSGQRRPRLVIEECPLTSEAQTNDAAKELIEKINVAAKRGMKFVGFISQHYSPSKFCNGTNHDGDIESMLHVRHGSDENCRSWNEGTLSGQSSESGIEEELHHESGQYQMEQNGSPTSSKSRKGEASDNKLYTVFNAFDDDSTSWAYQEGILSMKVTRKGSVISTLDADWLELTTFYYKQGLSLIDSFVFWETSKGEHLPKSLEGFFIYEEEGSGVPGSSRKGNDAIVVEQWTVIEGCEIKTDYGPLLHTLAEFGWLLTSVLPTPVLRHDSEGNLATKQIVFLQRPVMWNSAAQTPDKKASRHIKGEDKNKATSRSIGLDTTSSQPAESRHLPEECRLSPSRECWTKEGRLAQHNSFSGFSSSDNVLRELDDGQFDQEDGVTQVTCM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 raftlin family member 2
别名 C2orf11, Raft linking protein 2, Raftlin 2, raftlin family member 2, RFTN2
计算分子量 501 aa, 56 kDa
观测分子量 56-60 kDa
GenBank蛋白编号BC111069
基因名称 RFTN2
Gene ID (NCBI) 130132
RRIDAB_2300934
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ52LD8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for RFTN2 antibody 17854-1-APDownload protocol
WB protocol for RFTN2 antibody 17854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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