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验证数据展示

  • IHC staining of rat testis using 20207-1-AP

    IHC staining of rat testis using 20207-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20207-1-AP (RFPL4B-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 20207-1-AP

    IF Staining of A549 using 20207-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RFPL4B-Specific antibody (20207-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

RFPL4B-Specific Polyclonal antibody

RFPL4B-Specific Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 20207-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

IHC, IF/ICC, ELISA

别名

RFPL4B, RNF211, RING finger protein 211, Ret finger protein-like 4B, ret finger protein like 4B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20207-1-AP targets RFPL4B-Specific in IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ret finger protein-like 4B
别名 RFPL4B, RNF211, RING finger protein 211, Ret finger protein-like 4B, ret finger protein like 4B
计算分子量 30 kDa
GenBank蛋白编号NM_001013734
基因名称 RFPL4B
Gene ID (NCBI) 442247
RRIDAB_3669329
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZWI9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RFPL4B also named as RNF211. This antibody is specific to RFPL4B. RFPL4B may enable metal ion binding activity.

实验方案

Product Specific Protocols
IHC protocol for RFPL4B-Specific antibody 20207-1-APDownload protocol
IF protocol for RFPL4B-Specific antibody 20207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols