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RFK Polyclonal antibody

RFK Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 15813-1-AP

Print datasheet

Synonyms

Flavokinase, RFK, riboflavin kinase, RIFK

验证数据展示

  • WB analysis of HeLa using 15813-1-AP

    WB analysis of HeLa using 15813-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15813-1-AP (RFK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15813-1-AP

    IF Staining of HeLa using 15813-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15813-1-AP (RFK antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

15813-1-AP targets RFK in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen RFK fusion protein Ag8591 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name riboflavin kinase
Synonyms Flavokinase, RFK, riboflavin kinase, RIFK
Calculated Molecular Weight 162 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC007069
Gene Symbol RFK
Gene ID (NCBI) 55312
RRIDAB_2178612
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969G6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Riboflavin kinase (RFK) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis(PMID:12623014). RFK is rate-limiting in the synthesis of FAD, an essential prosthetic group of NADPH oxidase.

实验方案

Product Specific Protocols
WB protocol for RFK antibody 15813-1-APDownload protocol
IF protocol for RFK antibody 15813-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Transl Psychiatry

Long non-coding RNA Neat1 regulates adaptive behavioural response to stress in mice.

Authors - Michail S Kukharsky
View Article
mouseWB

Eur J Immunol

Riboflavin (vitamin B2 ) deficiency impairs Nox2 priming and defense against Listeria monocytogenes.

Authors - Schramm Michael M
View Article
mouseWB

Adv Sci (Weinh)

Biomimetic Remodeling of Microglial Riboflavin Metabolism Ameliorates Cognitive Impairment by Modulating Neuroinflammation

Authors - Mengran Zhang
View Article