验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
15813-1-AP targets RFK in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag8591 Product name: Recombinant human RFK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-162 aa of BC007069 Sequence: MPRADCIMRHLPYFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADISTGIYYGWASVGSGDVHKMVVSIGWNPYYKNTKKSMETHIMHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKRLELPEHLKIKEDNFFQVSKSKIMNGH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | riboflavin kinase |
| 别名 | ATP:riboflavin 5'-phosphotransferase, EC:2.7.1.26, Flavokinase, Riboflavin kinase, RIFK |
| 计算分子量 | 162 aa, 18 kDa |
| 观测分子量 | 15~ 18 kDa |
| GenBank蛋白编号 | BC007069 |
| 基因名称 | RFK |
| Gene ID (NCBI) | 55312 |
| RRID | AB_2178612 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q969G6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Riboflavin kinase (RFK) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis(PMID:12623014). RFK is rate-limiting in the synthesis of FAD, an essential prosthetic group of NADPH oxidase.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RFK antibody 15813-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Transl Psychiatry Long non-coding RNA Neat1 regulates adaptive behavioural response to stress in mice. | ||
Eur J Immunol Riboflavin (vitamin B2 ) deficiency impairs Nox2 priming and defense against Listeria monocytogenes. | ||
Adv Sci (Weinh) Biomimetic Remodeling of Microglial Riboflavin Metabolism Ameliorates Cognitive Impairment by Modulating Neuroinflammation | ||
Ecotoxicol Environ Saf Di (2-ethylhexyl) phthalate reduces sperm motility by decreasing sperm tail energy supply | ||
Redox Biol Riboflavin kinase binds and activates inducible nitric oxide synthase to reprogram macrophage polarization |
