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  • Featured Product
  • KD/KO Validated

RFFL Polyclonal antibody

RFFL Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 12687-1-AP

Print datasheet

Synonyms

CARP 2, Caspase regulator CARP2, Fring, RFFL, RIFIFYLIN, RING finger protein 189, RING finger protein 34 like, RNF189, RNF34L

验证数据展示

  • WB analysis of mouse testis using 12687-1-AP

    WB analysis of mouse testis using 12687-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12687-1-AP (RFFL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 12687-1-AP

    IP experiment of mouse testis using 12687-1-AP

    IP result of anti-RFFL (IP:12687-1-AP, 4ug; Detection:12687-1-AP 1:300) with mouse testis tissue lysate 3520 ug.

经过测试的应用

Positive WB detected inmouse testis tissue, mouse thymus tissue
Positive IP detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12687-1-AP targets RFFL in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RFFL fusion protein Ag3380 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger and FYVE-like domain containing 1
Synonyms CARP 2, Caspase regulator CARP2, Fring, RFFL, RIFIFYLIN, RING finger protein 189, RING finger protein 34 like, RNF189, RNF34L
Calculated Molecular Weight 327 aa, 37 kDa
Observed Molecular Weight 37-41 kDa
GenBank Accession NumberBC028424
Gene Symbol RFFL
Gene ID (NCBI) 117584
RRIDAB_10644289
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WZ73
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RFFL antibody 12687-1-APDownload protocol
IP protocol for RFFL antibody 12687-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome

Authors - Hana Nůsková
View Article
humanWB,CoIP

Cell Death Dis

ETS-1-activated LINC01016 over-expression promotes tumor progression via suppression of RFFL-mediated DHX9 ubiquitination degradation in breast cancers

Authors - Ying Sun
  • KD Validated
View Article