验证数据展示
经过测试的应用
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
10385-1-AP targets RFC5 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | RFC5 fusion protein Ag0584 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | replication factor C (activator 1) 5, 36.5kDa |
| 别名 | A1 36 kDa subunit, Activator 1 36 kDa subunit, Activator 1 subunit 5, Replication factor C subunit 5, RF C 36 kDa subunit, RFC36, RFC5 |
| 计算分子量 | 36 aa, 5 kDa |
| GenBank蛋白编号 | BC001866 |
| 基因名称 | RFC5 |
| Gene ID (NCBI) | 5985 |
| RRID | AB_2178603 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P40937 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C(RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. Replication factor C5 (RFC5, synonyms: RFC36, MGC1155) is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA and form a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for RFC5 antibody 10385-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
bioRxiv Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination | ||
Cell Rep Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination | ||
Am J Hum Genet Expanding the genetic and phenotypic landscape of replication factor C complex-related disorders: RFC4 deficiency is linked to a multisystemic disorder |