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验证数据展示

  • WB analysis of Jurkat using 19159-1-AP

    WB analysis of Jurkat using 19159-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19159-1-AP (RFC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 19159-1-AP

    WB analysis of HeLa using 19159-1-AP

    WB result of RFC1 antibody (19159-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RFC1 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

RFC1 Polyclonal antibody

RFC1 Polyclonal Antibody for WB, ELISA
Cat No. 19159-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

DNA binding protein PO GA, Activator 1 subunit 1, Activator 1 large subunit, Activator 1 140 kDa subunit, A1 140 kDa subunit

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19159-1-AP targets RFC1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13585

Product name: Recombinant human RFC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 888-1147 aa of BC051751

Sequence: YIHVKPVAAGGDMKKHLMLLSRAADSICDGDLVDSQIRSKQNWSLLPAQAIYASVLPGELMRGYMTQFPTFPSWLGKHSSTGKHDRIVQDLALHMSLRTYSSKRTVNMDYLSLLRDALVQPLTSQGVDGVQDVVALMDTYYLMKEDFENIMEISSWGGKPSPFSKLDPKVKAAFTRAYNKEAHLTPYSLQAIKASRHSTSPSLDSEYNEELNEDDSQSDEKDQDAIETDAMIKKKTKSSKPSKPEKDKEPRKGKGKSSKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 replication factor C (activator 1) 1, 145kDa
别名 DNA binding protein PO GA, Activator 1 subunit 1, Activator 1 large subunit, Activator 1 140 kDa subunit, A1 140 kDa subunit
计算分子量 128 kDa
观测分子量 128-150 kDa
GenBank蛋白编号BC051751
基因名称 RFC1
Gene ID (NCBI) 5981
RRIDAB_2878574
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35251
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Replication factor C subunit 1 (RFC1) is a pentameric DNA polymerase accessory protein involved in DNA replication and repair (PMID: 10051561). It was reported as a cause of cerebellar ataxia with neuropathy and vestibular areflexia syndrome (CANVAS) (PMID: 30926972).

实验方案

Product Specific Protocols
WB protocol for RFC1 antibody 19159-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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