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REV1 Polyclonal antibody

REV1 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17703-1-AP

Print datasheet

Synonyms

AIBP80, DNA repair protein REV1, REV1, REV1 homolog (S. cerevisiae), REV1L

验证数据展示

  • WB analysis using 17703-1-AP

    WB analysis using 17703-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17703-1-AP (REV1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 17703-1-AP

    IF Staining of U2OS using 17703-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using REV1 antibody (17703-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells
Positive IF detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17703-1-AP targets REV1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen REV1 fusion protein Ag11975 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name REV1 homolog (S. cerevisiae)
Synonyms AIBP80, DNA repair protein REV1, REV1, REV1 homolog (S. cerevisiae), REV1L
Calculated Molecular Weight 1114 aa, 122 kDa
Observed Molecular Weight140-150 kDa
GenBank Accession NumberBC037734
Gene Symbol REV1
Gene ID (NCBI) 51455
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBZ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rev1 is a member of the TLS polymerase family and plays a key role in this mutagenic pathway, which allows the bypass of modified DNA bases and respectively, facilitates proliferation even in the presence of extensive DNA damage, such as during chemotherapy. Mutations in human REV1 have been detected in a minority of tumors, and single-nucleotide polymorphisms (SNPs) in the hREV1 gene have been linked to various types of human cancer.

实验方案

Product Specific Protocols
WB protocol for REV1 antibody 17703-1-APDownload protocol
IF protocol for REV1 antibody 17703-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols