验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16895-1-AP targets RETSAT in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag10420 Product name: Recombinant human RETSAT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC011418 Sequence: MYSGEKAYIQGLKEKFPQEEAIIDKYIKLVKVVSSGAPHAILLKFLPLPVVQLLDRCGLLTRFSPFLQASTQSLAEVLQQLGASSELQAVLSYIFPTYGVTPNHSAFSMHALLVNHYMKGGFYPRGGSSQIAFHTIPVIQRAGGAVLTKATVQSVLLDSAGKACGVSVKKGHELVNIYCPIVVSNAGLFNTYEHLLPGNARCLPGVKQQLGTVRPGLGMTSVFICLRGTKEDLHLPSTNYYVYYDTDMDQAMERYVSMPREEAAEHIPLLFFAFPSAKDPTWEDRFPGGECDCRIPTHQPVLSGCSPRCLLRG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | retinol saturase (all-trans-retinol 13,14-reductase) |
| 别名 | All-trans-13,14-dihydroretinol saturase, All-trans-retinol 13,14-reductase, EC:1.3.99.23, PPAR-alpha-regulated and starvation-induced gene protein, PPSIG |
| 计算分子量 | 610 aa, 69 kDa |
| 观测分子量 | 62-67 kDa |
| GenBank蛋白编号 | BC011418 |
| 基因名称 | RETSAT |
| Gene ID (NCBI) | 54884 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6NUM9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RETSAT (Retinol Saturase) is an enzyme-encoding gene that plays a critical role in cellular metabolism, primarily involved in the saturation of retinol (vitamin A) and other molecules. It catalyzes the conversion of all-trans-retinol to all-trans-13,14-dihydroretinol, a process that links vitamin A metabolism to lipid and glucose homeostasis. It is highly expressed in the liver, adipose tissue, and kidney and is regulated by the PPARγ master regulator of adipogenesis (PMID: 28855500; 38128827). RETSAT also has a role in cancer cell survival, particularly in hypoxic tumor environments, by enhancing resistance to replication stress (PMID: 36109793).
