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验证数据展示

  • WB analysis using 29330-1-AP

    WB analysis using 29330-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29330-1-AP (Renin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 29330-1-AP

    IF Staining of HepG2 using 29330-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Renin antibody (29330-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Renin Polyclonal antibody

Renin Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29330-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, monkey

应用

WB, IF/ICC, ELISA

别名

REN, EC:3.4.23.15, Angiotensinogenase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOS-7 cells, HepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29330-1-AP targets Renin in WB, IF/ICC, ELISA applications and shows reactivity with human, monkey samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, monkey
免疫原

CatNo: Ag31071

Product name: Recombinant human REN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 112-235 aa of BC047752

Sequence: VPSSKCSRLYTACVYHKLFDASDSSSYKHNGTELTLRYSTGTVSGFLSQDIITVGGITVTQMFGEVTEMPALPFMLAEFDGVVGMGFIEQAIGRVTPIFDNIISQGVLKEDVFSFYYNRDSENS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 renin
别名 REN, EC:3.4.23.15, Angiotensinogenase
计算分子量 45 kDa
观测分子量45 kDa
GenBank蛋白编号BC047752
基因名称 Renin
Gene ID (NCBI) 5972
RRIDAB_3086119
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP00797
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Renin is an aspartic protease that consists of 2 homologous lobes, produced as pre-prorenin protein and transferred into the cisterns of the endoplasmic reticulum. Renin is mainly produced and released into circulation by the so-called juxtaglomerular epithelioid cells, located in the walls of renal afferent arterioles at the entrance of the glomerular capillary network (PMID: 16525949).REN has 2 isoforms produced by alternative splicing with a molecular weight of 45-47kDa.

实验方案

Product Specific Protocols
IF protocol for Renin antibody 29330-1-APDownload protocol
WB protocol for Renin antibody 29330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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