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  • Featured Product
  • KD/KO Validated

REEP6 Polyclonal antibody

REEP6 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 12088-1-AP

Print datasheet

Synonyms

C19orf32, DP1L1, receptor accessory protein 6, REEP6, TB2L1

验证数据展示

  • WB analysis of BxPC-3 using 12088-1-AP

    WB analysis of BxPC-3 using 12088-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12088-1-AP (REEP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse olfactory epithelium tissue using 12088-1-AP

    IF Staining of mouse olfactory epithelium tissue using 12088-1-AP

    Fluorescent IHC on 1%PLP fixed frozen mouse olfactory epithelium tissue of REEP6 antibody (12088-1-AP, 1:100). Excellent staining of Sus cells on the apical layer as well as their basal projections. By Dr. Brian Lin (Schwob Lab).

  • IHC staining of human pancreas cancer using 12088-1-AP

    IHC staining of human pancreas cancer using 12088-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12088-1-AP (REEP6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 12088-1-AP

    IHC staining of human pancreas cancer using 12088-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12088-1-AP (REEP6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inBxPC-3 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse olfactory epithelium tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12088-1-AP targets REEP6 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsWB, IP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen REEP6 fusion protein Ag2723 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name receptor accessory protein 6
Synonyms C19orf32, DP1L1, receptor accessory protein 6, REEP6, TB2L1
Calculated Molecular Weight 201 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC008201
Gene Symbol REEP6
Gene ID (NCBI) 92840
RRIDAB_2178460
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96HR9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for REEP6 antibody 12088-1-APDownload protocol
IHC protocol for REEP6 antibody 12088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIP

Mol Psychiatry

Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease.

Authors - M G Sharoar
View Article
ratWB

J Clin Invest

Nasal neuron PET imaging quantifies neuron generation and degeneration.

Authors - Genevieve C Van de Bittner
View Article
humanWB,IF

Am J Hum Genet

Mutations in REEP6 Cause Autosomal-Recessive Retinitis Pigmentosa.

Authors - Gavin Arno
View Article
mouseIF

Hum Mol Genet

REEP6 deficiency leads to retinal degeneration through disruption of ER homeostasis and protein trafficking.

Authors - Smriti A Agrawal
View Article