RECQL5 Polyclonal antibody
RECQL5 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, mouse testis tissue |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12468-2-AP targets RECQL5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Tested Reactivity | human, mouse, rat |
Immunogen | RECQL5 fusion protein Ag3145 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | RecQ protein-like 5 |
Synonyms | ATP dependent DNA helicase Q5, DNA helicase, RecQ like type 5, RecQ protein like 5, RECQ5, RECQL5 |
Calculated Molecular Weight | 435 aa, 49 kDa, 409 kDa |
Observed Molecular Weight | 48 kDa, 130-140 kDa |
GenBank Accession Number | BC016911 |
Gene Symbol | RECQL5 |
Gene ID (NCBI) | 9400 |
RRID | AB_2300899 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O94762 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for RECQL5 antibody 12468-2-AP | Download protocol |
IHC protocol for RECQL5 antibody 12468-2-AP | Download protocol |
IF protocol for RECQL5 antibody 12468-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |