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验证数据展示

  • WB analysis using 12468-2-AP

    WB analysis using 12468-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12468-2-AP (RECQL5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 12468-2-AP

    IHC staining of human pancreas cancer using 12468-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12468-2-AP (RECQL5 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with RECQL5 antibody included
  • IF Staining of HepG2 using 12468-2-AP

    IF Staining of HepG2 using 12468-2-AP

    Immunofluorescent analysis of HepG2 cells, using RECQL5 antibody 12468-2-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

RECQL5 Polyclonal antibody

RECQL5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12468-2-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

EC:5.6.2.4, DNA helicase, RecQ-like type 5, DNA helicase, RecQ like type 5, DNA 3'-5' helicase RecQ5, ATP-dependent DNA helicase Q5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, mouse testis tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RECQL5 Ready-To-Use IHC Kit. RECQL5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12468-2-AP targets RECQL5 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag3145

Product name: Recombinant human RECQL5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC016911

Sequence: MSSHHTTFPFDPERRVRSTLKKVFGFDSFKTPLQESATMAVVKGNKDVFVCMPTGAGKSLCYQLPALLAKGITIVVSPLIALIQDQVDHLLTLKVRVSSLNSKLSAQERKELLADLEREKPQTKILYITPEMAASSSFQPTLNSLVSRHLLSYLVVDEAHCVSQWGHDFRPDYLRLGALRSRLGHAPCVALTATATPQVQEDVFAALHLKKPVAIFKTPCFRANLFYDVQFKELISDPYGNLKDFCLKALGQEADKGLSGCGIVYCRTREACEQLAIELSCRGVNAKAYHAGLKASERTLVQNDWMEEKVPVIVATISFGMGVDKANVRFVAHWNIAKSMAGYYQESGRA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RecQ protein-like 5
别名 EC:5.6.2.4, DNA helicase, RecQ-like type 5, DNA helicase, RecQ like type 5, DNA 3'-5' helicase RecQ5, ATP-dependent DNA helicase Q5
计算分子量 435 aa, 49 kDa, 409 kDa
观测分子量 48 kDa, 130-140 kDa
GenBank蛋白编号BC016911
基因名称 RECQL5
Gene ID (NCBI) 9400
RRIDAB_2300899
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94762
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RECQL5 antibody 12468-2-APDownload protocol
IHC protocol for RECQL5 antibody 12468-2-APDownload protocol
IF protocol for RECQL5 antibody 12468-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,CoIP

bioRxiv

Senataxin and DNA-PKcs Redundantly Promote Non-Homologous End Joining Repair of DNA Double Strand Breaks During V(D)J Recombination

Authors - Bo-Ruei Chen
View Article
humanWB

Adv Sci (Weinh)

Specific Monitoring the DNA Helicase Function via Anchor-Embedded DNA Probe

Authors - Keni Ning
View Article
humanWB

bioRxiv

Distinct mechanisms underlying extrachromosomal telomere DNA generation in ALT cancers

Authors - Junyeop Lee
View Article