验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10793-1-AP targets REC8 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | REC8 fusion protein Ag1250 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | REC8 homolog (yeast) |
| 别名 | Cohesin Rec8p, HR21spB, REC8, REC8 homolog (yeast), REC8L1, Rec8p |
| 计算分子量 | 63 kDa |
| 观测分子量 | 85-95 kDa |
| GenBank蛋白编号 | BC010887 |
| 基因名称 | REC8 |
| Gene ID (NCBI) | 9985 |
| RRID | AB_2178282 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95072 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
REC8, also named as Rec8p and REC8L1, belongs to the rad21 family. This cohesin protein is required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. Rec8 is present in an unphosphorylated form early in meiotic prophase but is phosphorylated prior to meiosis I. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human REC8. This antibody can recognize the monomeric, non-phosphorylated form(63-70kd) of REC8 and other forms(80-100kd) which represent either posttranslationally modified forms or alternative transcript products (PMID: 12615909,19463812).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for REC8 antibody 10793-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Cancer Res Ectopic Expression of Cancer-Testis Antigens in Cutaneous T-cell Lymphoma Patients. | ||
Cell Cycle Elevated intracellular pH appears in aged oocytes and causes oocyte aneuploidy associated with the loss of cohesion in mice. | ||
Biomed Pharmacother Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway. | ||
Int J Mol Sci "Mitotic Slippage" and Extranuclear DNA in Cancer Chemoresistance: A Focus on Telomeres. | ||
Exp Cell Res The role of meiotic cohesin REC8 in chromosome segregation in gamma irradiation-induced endopolyploid tumour cells. |
