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RDH5 Polyclonal antibody

RDH5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 13140-1-AP

Print datasheet

Synonyms

11 cis RDH, 11 cis retinol dehydrogenase, HSD17B9, RDH1, RDH5, SDR9C5

验证数据展示

  • WB analysis using 13140-1-AP

    WB analysis using 13140-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13140-1-AP (RDH5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse eye tissue, rat eye tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13140-1-AP targets RDH5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen RDH5 fusion protein Ag3784 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name retinol dehydrogenase 5 (11-cis/9-cis)
Synonyms 11 cis RDH, 11 cis retinol dehydrogenase, HSD17B9, RDH1, RDH5, SDR9C5
Calculated Molecular Weight 318 aa, 35 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC028298
Gene Symbol RDH5
Gene ID (NCBI) 5959
RRIDAB_2918031
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92781
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RDH5, also named as RDH1, SDR9C5 and HSD17B9, belongs to short-chain dehydrogenases/reductases (SDR) family. RDH5 catalyzes the oxidation of cis-isomers of retinol in an NAD-dependent manner for the final step in the biosynthesis of 11-cis retinaldehyde. Mutations in this gene are associated with fundus albipunctatus, an autosomal recessive eye disease characterized by stationary night blindness and accumulation of white spots in the retina (PubMed:11675386). The predicted size of this protein is 35 kDa. Some paper have reported that the molecular weight is 32 kDa and could form a dimer of about 60 kDa.

实验方案

Product Specific Protocols
WB protocol for RDH5 antibody 13140-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols