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验证数据展示

  • WB analysis of human heart using 16283-1-AP

    WB analysis of human heart using 16283-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16283-1-AP (RDH14 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16283-1-AP

    IHC staining of human kidney using 16283-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16283-1-AP (RDH14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 16283-1-AP

    IHC staining of human kidney using 16283-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16283-1-AP (RDH14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RDH14 Polyclonal antibody

RDH14 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16283-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Short chain dehydrogenase/reductase family 7C member 4, SDR7C4, Retinol dehydrogenase 14, PAN2, EC:1.1.1.300

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue, A549 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16283-1-AP targets RDH14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RDH14 fusion protein Ag9386 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 retinol dehydrogenase 14 (all-trans/9-cis/11-cis)
别名 Short chain dehydrogenase/reductase family 7C member 4, SDR7C4, Retinol dehydrogenase 14, PAN2, EC:1.1.1.300
计算分子量 336 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC009830
基因名称 RDH14
Gene ID (NCBI) 57665
RRIDAB_2158906
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HBH5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RDH14 antibody 16283-1-APDownload protocol
IHC protocol for RDH14 antibody 16283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols