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验证数据展示

  • WB analysis of human heart using 16283-1-AP

    WB analysis of human heart using 16283-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16283-1-AP (RDH14 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16283-1-AP

    IHC staining of human kidney using 16283-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16283-1-AP (RDH14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 16283-1-AP

    IHC staining of human kidney using 16283-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16283-1-AP (RDH14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RDH14 Polyclonal antibody

RDH14 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16283-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Short chain dehydrogenase/reductase family 7C member 4, SDR7C4, Retinol dehydrogenase 14, PAN2, EC:1.1.1.300

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman heart tissue, A549 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16283-1-AP targets RDH14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9386

Product name: Recombinant human RDH14 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-335 aa of BC009830

Sequence: MAVATAAAVLAALGGALWLAARRFVGPRVQRLRRGGDPGLMHGKTVLITGANSGLGRATAAELLRLGARVIMGCRDRARAEEAAGQLRRELRQAAECGPEPGVSGVGELIVRELDLASLRSVRAFCQEMLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQFGVNHLGHFLLTNLLLGLLKSSAPSRIVVVSSKLYKYGDINFDDLNSEQSYNKSFCYSRSKLANILFTRELARRLEGTNVTVNVLHPGIVRTNLGRHIHIPLLVKPLFNLVSWAFFKTPVEGAQTSIYLASSPEVEGVSGRYFGDCKEEELLPKAMDESVARKLWDISEVMVGLL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 retinol dehydrogenase 14 (all-trans/9-cis/11-cis)
别名 Short chain dehydrogenase/reductase family 7C member 4, SDR7C4, Retinol dehydrogenase 14, PAN2, EC:1.1.1.300
计算分子量 336 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC009830
基因名称 RDH14
Gene ID (NCBI) 57665
RRIDAB_2158906
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HBH5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RDH14 antibody 16283-1-APDownload protocol
IHC protocol for RDH14 antibody 16283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols