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验证数据展示

  • WB analysis using 13289-3-AP

    WB analysis using 13289-3-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 13289-3-AP (RDH12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 13289-3-AP

    IHC staining of mouse eye using 13289-3-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13289-3-AP (RDH12 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse eye using 13289-3-AP

    IHC staining of mouse eye using 13289-3-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13289-3-AP (RDH12 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RDH12 Polyclonal antibody

RDH12 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13289-3-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

LCA13, LCA3, RDH12, Retinol dehydrogenase 12, SDR7C2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse eye tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13289-3-AP targets RDH12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RDH12 fusion protein Ag3850 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 retinol dehydrogenase 12 (all-trans/9-cis/11-cis)
别名 LCA13, LCA3, RDH12, Retinol dehydrogenase 12, SDR7C2
计算分子量 316 aa, 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC025724
基因名称 RDH12
Gene ID (NCBI) 145226
RRIDAB_2178131
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96NR8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RDH12 antibody 13289-3-APDownload protocol
IHC protocol for RDH12 antibody 13289-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols