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RDH12 Polyclonal antibody

RDH12 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13289-3-AP

Print datasheet

Synonyms

LCA13, LCA3, RDH12, Retinol dehydrogenase 12, SDR7C2

验证数据展示

  • WB analysis using 13289-3-AP

    WB analysis using 13289-3-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 13289-3-AP (RDH12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 13289-3-AP

    IHC staining of mouse eye using 13289-3-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13289-3-AP (RDH12 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse eye using 13289-3-AP

    IHC staining of mouse eye using 13289-3-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13289-3-AP (RDH12 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse eye tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13289-3-AP targets RDH12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen RDH12 fusion protein Ag3850 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name retinol dehydrogenase 12 (all-trans/9-cis/11-cis)
Synonyms LCA13, LCA3, RDH12, Retinol dehydrogenase 12, SDR7C2
Calculated Molecular Weight 316 aa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC025724
Gene Symbol RDH12
Gene ID (NCBI) 145226
RRIDAB_2178131
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96NR8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RDH12 antibody 13289-3-APDownload protocol
IHC protocol for RDH12 antibody 13289-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols