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验证数据展示

  • IHC staining of human prostate cancer using 11077-1-AP

    IHC staining of human prostate cancer using 11077-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11077-1-AP (RDH11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RDH11 antibody included
  • IF Staining of HaCaT using 11077-1-AP

    IF Staining of HaCaT using 11077-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using RDH11 antibody (11077-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

RDH11 Polyclonal antibody

RDH11 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 11077-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA

别名

Prostate short-chain dehydrogenase/reductase 1, HCV core-binding protein HCBP12, EC:1.1.1.300, CGI-82, ARSDR1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells
Planning an IHC experiment? We recommend our IHCeasy RDH11 Ready-To-Use IHC Kit. RDH11 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11077-1-AP targets RDH11 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RDH11 fusion protein Ag1485 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 retinol dehydrogenase 11 (all-trans/9-cis/11-cis)
别名 Prostate short-chain dehydrogenase/reductase 1, HCV core-binding protein HCBP12, EC:1.1.1.300, CGI-82, ARSDR1
计算分子量 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC011727
基因名称 RDH11
Gene ID (NCBI) 51109
RRIDAB_2177870
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TC12
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RDH11(Retinol dehydrogenase 11) is also named as ARSDR1(androgen-regulated short-chain dehydrogenase/reductase 1 ), PSDR1(prostate short-chain dehydrogenase/reductase 1) and belongs to the short-chain dehydrogenases/reductases (SDR) family. RDH11 is involved in steroid synthesis and/or degradation in normal and neoplastic prostate epithelium, and, as such, it may be a key enzyme involved in maintaining intracellular balance of steroid hormones in these cells(PMID:11245473). The deduced protein contains 318 amino acids, and the transcript contains 2 potential polyadenylation signals. It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IHC protocol for RDH11 antibody 11077-1-APDownload protocol
IF protocol for RDH11 antibody 11077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols