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验证数据展示

  • WB analysis using 84956-4-RR (same clone as 84956-4-PBS)

    WB analysis using 84956-4-RR (same clone as 84956-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84956-4-RR (RCN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 84956-4-RR (same clone as 84956-4-PBS)

    IHC staining of mouse testis using 84956-4-RR (same clone as 84956-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84956-4-RR (RCN2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RCN2 antibody included
  • IF Staining of HeLa using 84956-4-RR (same clone as 84956-4-PBS)

    IF Staining of HeLa using 84956-4-RR (same clone as 84956-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RCN2 antibody (84956-4-RR, Clone: 242612A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84956-4-RR (same clone as 84956-4-PBS)

    IF Staining of HepG2 using 84956-4-RR (same clone as 84956-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RCN2 antibody (84956-4-RR, Clone: 242612A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

RCN2 Recombinant monoclonal antibody, PBS Only

RCN2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84956-4-PBS

产品说明书

CloneNo. 242612A4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

242612A4, Calcium binding protein ERC 55, Calcium-binding protein ERC-55, E6 binding protein, E6-binding protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RCN2 Ready-To-Use IHC Kit. RCN2 primary antibody included.

产品信息

84956-4-PBS targets RCN2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0247

Product name: Recombinant human RCN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-209 aa of BC004892

Sequence: GKAEELHYPLGERRSDYDREALLGVQEDVDEYVKLGHEEQQKRLQAIIKKIDLDSDGFLTESELSSWIQMSFKHYAMQEAKQQFVEYDKNSDDTVTWDEYNIQMYDRVIDFDENTALDDAEEESFRKLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 reticulocalbin 2, EF-hand calcium binding domain
别名 242612A4, Calcium binding protein ERC 55, Calcium-binding protein ERC-55, E6 binding protein, E6-binding protein
计算分子量 37 kDa
观测分子量 55 kDa
GenBank蛋白编号BC004892
基因名称 RCN2
Gene ID (NCBI) 5955
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14257
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Reticulocalbin-2 (RCN2) is also named as endoplasmic reticulum Ca2+-binding protein of 55 kDa (ERC55) and E6-binding protein (E6BP). It is strictly located in endoplasmic reticulum (PMID: 34094681). RCN2 is a low-affinity Ca2+ binding protein localized to the secretory pathway of mammalian cells, belongs to a member of the EF-hand Ca2+ binding protein family, which participate in the secretory process, chaperone activity, signal transduction as well as participation in a large variety of disease processes (PMID: 19260022). As for cancer research, several studies showed that RCN2 was related to tumor progression, such as mammary cancer, cervical cancer, neck squamous cell carcinoma and colorectal cancer (PMID: 16351756, PMID: 32422974). RCN2 played a pivotal role in HCC pathogenesis through regulating activation of the epidermal growth factor receptor (EGFR) pathway and modulating epidermal growth factor receptor EGFR dimerization and internalization (PMID: 28745317).

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