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验证数据展示

  • WB analysis using 10193-2-AP (same clone as 10193-2-PBS)

    WB analysis using 10193-2-AP (same clone as 10193-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 10193-2-AP (same clone as 10193-2-PBS)

    IHC staining of human gliomas using 10193-2-AP (same clone as 10193-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RCN2 antibody included
  • IF Staining of HeLa using 10193-2-AP (same clone as 10193-2-PBS)

    IF Staining of HeLa using 10193-2-AP (same clone as 10193-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RCN2 antibody (10193-2-AP) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 10193-2-AP (same clone as 10193-2-PBS)

    IF Staining of HepG2 using 10193-2-AP (same clone as 10193-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RCN2 antibody (10193-2-AP) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • IP experiment of mouse lung using 10193-2-AP (same clone as 10193-2-PBS)

    IP experiment of mouse lung using 10193-2-AP (same clone as 10193-2-PBS)

    IP result of anti-RCN2 (IP:10193-2-AP, 4ug; Detection:10193-2-AP 1:400) with mouse lung tissue lysate 2800ug. This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RCN2 Polyclonal antibody, PBS Only

RCN2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10193-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Calcium binding protein ERC 55, Calcium-binding protein ERC-55, E6 binding protein, E6-binding protein, E6BP

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RCN2 Ready-To-Use IHC Kit. RCN2 primary antibody included.

产品信息

10193-2-PBS targets RCN2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0247

Product name: Recombinant human RCN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 24-209 aa of BC004892

Sequence: KAEELHYPLGERRSDYDREALLGVQEDVDEYVKLGHEEQQKRLQAIIKKIDLDSDGFLTESELSSWIQMSFKHYAMQEAKQQFVEYDKNSDDTVTWDEYNIQMYDRVIDFDENTALDDAEEESFRKLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 reticulocalbin 2, EF-hand calcium binding domain
别名 Calcium binding protein ERC 55, Calcium-binding protein ERC-55, E6 binding protein, E6-binding protein, E6BP
计算分子量 37 kDa
观测分子量 55 kDa
GenBank蛋白编号BC004892
基因名称 RCN2
Gene ID (NCBI) 5955
RRIDAB_2180018
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14257
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Reticulocalbin 2 (RCN2) is a Ca(2+)-binding protein that is specifically localized in the endoplasmic reticulum (ER). It is also termed ERC-55, i.e. ER calcium-binding protein of 55 kDa. It is a single copy gene and is encoded by an approximately 1900-base mRNA, which shows a ubiquitous expression pattern. The protein comprises an amino-terminal signal sequence followed by six copies of the EF-hand Ca2+ binding motif.

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