验证数据展示
经过测试的应用
| Positive WB detected in | mouse spleen tissue, HL-60 cells, rat testis tissue, mouse testis tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
13820-1-AP targets Pirh2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag4916 Product name: Recombinant human RCHY1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-261 aa of BC047393 Sequence: MAATAREDGASGQERGQRGCEHYDRGCLLKAPCCDKLYTCRLCHDNNEDHQLDRFKVKEVQCINCEKIQHAQQTCEECSTLFGEYYCDICHLFDKDKKQYHCENCGICRIGPKEDFFHCLKCNLCLAMNLQGRHKCIENVSQQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLKEGYRCPLCMHSALDMTRYWRQLDDEVAQTPMPSEYQNMTVDILCNDCNGRSTVQFHILGMKCKICESYNTAQAGGRRISLDQQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ring finger and CHY zinc finger domain containing 1 |
| 别名 | RCHY1, EC:2.3.2.27, E3 ubiquitin-protein ligase Pirh2, CH-rich-interacting match with PLAG1, CHIMP |
| 计算分子量 | 261 aa, 30 kDa |
| 观测分子量 | 45 KDa, 30 kDa |
| GenBank蛋白编号 | BC047393 |
| 基因名称 | RCHY1 |
| Gene ID (NCBI) | 25898 |
| RRID | AB_2891219 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96PM5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Pirh2 is also named as RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363 and has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. Pirh2 monoubiquitinates the translesion DNA polymerase POLH and contributes to the regulation of the cell cycle progression. This protein has 8 isoforms produced by alternative splicing and can exsit as a monomer or homodimer.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Pirh2 antibody 13820-1-AP | Download protocol |
| WB protocol for Pirh2 antibody 13820-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.
| ||
Sci Adv G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis. | ||
J Agric Food Chem Avenanthramide A induces cellular senescence via miR-129-3p/Pirh2/p53 signaling pathway to suppress colon cancer growth.
|
