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RCCD1 Polyclonal antibody

RCCD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 24446-1-AP

Print datasheet

Synonyms

RCC1 domain containing 1, RCCD1

验证数据展示

  • WB analysis using 24446-1-AP

    WB analysis using 24446-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24446-1-AP (RCCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24446-1-AP targets RCCD1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA
Tested Reactivity human
Immunogen RCCD1 fusion protein Ag19685 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RCC1 domain containing 1
Synonyms RCC1 domain containing 1, RCCD1
Calculated Molecular Weight 376 aa, 40 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC140708
Gene Symbol RCCD1
Gene ID (NCBI) 91433
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA6NED2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RCCD1 is a new member of the large, phylogenetically conserved, RCC1 superfamily. Many RCC1 superfamily proteins have been linked to several human diseases, including cancer. RCCD1 was reported as a partner of histone H3K36 demethylase KDM8 involved in chromosome segregation, and has been identified as a potential driver for breast cancer. RCCD1 plays a role in regulating alpha-tubulin deacetylation and cytoskeletal microtubule stability, thereby promoting cell migration and TGF-beta-induced epithelial-to-mesenchymal transition (EMT), potentially through the inhibition of KDM8.

实验方案

Product Specific Protocols
WB protocol for RCCD1 antibody 24446-1-APDownload protocol
IHC protocol for RCCD1 antibody 24446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols