验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells, Jurkat cells |
Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
24446-1-AP targets RCCD1 in WB, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human |
免疫原 | RCCD1 fusion protein Ag19685 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | RCC1 domain containing 1 |
别名 | RCC1 domain-containing protein 1, RCC1 domain containing 1 |
计算分子量 | 376 aa, 40 kDa |
观测分子量 | 40 kDa |
GenBank蛋白编号 | BC140708 |
基因名称 | RCCD1 |
Gene ID (NCBI) | 91433 |
RRID | AB_3085734 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | A6NED2 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RCCD1 is a new member of the large, phylogenetically conserved, RCC1 superfamily. Many RCC1 superfamily proteins have been linked to several human diseases, including cancer. RCCD1 was reported as a partner of histone H3K36 demethylase KDM8 involved in chromosome segregation, and has been identified as a potential driver for breast cancer. RCCD1 plays a role in regulating alpha-tubulin deacetylation and cytoskeletal microtubule stability, thereby promoting cell migration and TGF-beta-induced epithelial-to-mesenchymal transition (EMT), potentially through the inhibition of KDM8.
实验方案
Product Specific Protocols | |
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WB protocol for RCCD1 antibody 24446-1-AP | Download protocol |
IHC protocol for RCCD1 antibody 24446-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |