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验证数据展示

  • WB analysis using 24446-1-AP

    WB analysis using 24446-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24446-1-AP (RCCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 24446-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 24446-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24446-1-AP (RCCD1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 24446-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 24446-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24446-1-AP (RCCD1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RCCD1 Polyclonal antibody

RCCD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24446-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

RCC1 domain-containing protein 1, RCC1 domain containing 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24446-1-AP targets RCCD1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 RCCD1 fusion protein Ag19685 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RCC1 domain containing 1
别名 RCC1 domain-containing protein 1, RCC1 domain containing 1
计算分子量 376 aa, 40 kDa
观测分子量40 kDa
GenBank蛋白编号BC140708
基因名称 RCCD1
Gene ID (NCBI) 91433
RRIDAB_3085734
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA6NED2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RCCD1 is a new member of the large, phylogenetically conserved, RCC1 superfamily. Many RCC1 superfamily proteins have been linked to several human diseases, including cancer. RCCD1 was reported as a partner of histone H3K36 demethylase KDM8 involved in chromosome segregation, and has been identified as a potential driver for breast cancer. RCCD1 plays a role in regulating alpha-tubulin deacetylation and cytoskeletal microtubule stability, thereby promoting cell migration and TGF-beta-induced epithelial-to-mesenchymal transition (EMT), potentially through the inhibition of KDM8.

实验方案

Product Specific Protocols
WB protocol for RCCD1 antibody 24446-1-APDownload protocol
IHC protocol for RCCD1 antibody 24446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols