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验证数据展示

  • WB analysis using 67335-1-Ig (same clone as 67335-1-PBS)

    WB analysis using 67335-1-Ig (same clone as 67335-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67335-1-Ig (RCC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67335-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67335-1-Ig (same clone as 67335-1-PBS)

    IHC staining of mouse brain using 67335-1-Ig (same clone as 67335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67335-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RCC1 antibody included

RCC1 Monoclonal antibody, PBS Only

RCC1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67335-1-PBS

产品说明书

CloneNo. 2C2F5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse

应用

WB, IHC, Indirect ELISA

别名

Cell cycle regulatory protein, CHC1, RCC1, RCC1 I

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RCC1 Ready-To-Use IHC Kit. RCC1 primary antibody included.

产品信息

67335-1-PBS targets RCC1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, mouse
免疫原

CatNo: Ag17809

Product name: Recombinant human RCC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-421 aa of BC010067

Sequence: MSPKRIAKRRSPPADAIPKSKKVKVSHRSHSTEPGLVLTLGQGDVGQLGLGENVMERKKPALVSIPEDVVQAEAGGMHTVCLSKSGQVYSFGCNDEGALGRDTSVEGSEMVPGKVELQEKVVQVSAGDSHTAALTDDGRVFLWGSFRDNNGVIGLLEPMKKSMVPVQVQLDVPVVKVASGNDHLVMLTADGDLYTLGCGEQGQLGRVPELFANRGGRQGLERLLVPKCVMLKSRGSRGHVRFQDAFCGAYFTFAISHEGHVYGFGLSNYHQLGTPGTESCFIPQNLTSFKNSTKSWVGFSGGQHHTVCMDSEGKAYSLGRAEYGRLGLGEGAEEKSIPTLISRLPAVSSVACGASVGYAVTKDGRVFAWGMGTNYQLGTGQDEDAWSPVEMMGKQLENRVVLSVSSGGQHTVLLVKDKEQS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 regulator of chromosome condensation 1
别名 Cell cycle regulatory protein, CHC1, RCC1, RCC1 I
计算分子量 421 aa, 45 kDa
观测分子量45-50 kDa
GenBank蛋白编号BC010067
基因名称 RCC1
Gene ID (NCBI) 1104
RRIDAB_2882593
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP18754
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation.

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