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RCC1 Polyclonal antibody

RCC1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 22335-1-AP

Print datasheet

Synonyms

Cell cycle regulatory protein, CHC1, RCC1, RCC1 I

验证数据展示

  • WB analysis using 22335-1-AP

    WB analysis using 22335-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22335-1-AP (RCC1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 22335-1-AP

    IP experiment of HeLa using 22335-1-AP

    IP result of anti-RCC1 (IP:22335-1-AP, 3ug; Detection:22335-1-AP 1:1500) with HeLa cells lysate 3000ug.

  • IHC staining of mouse testis using 22335-1-AP

    IHC staining of mouse testis using 22335-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 22335-1-AP

    IHC staining of mouse testis using 22335-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 22335-1-AP

    IF Staining of HepG2 using 22335-1-AP

    Immunofluorescent analysis of HepG2 cells using 22335-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inSH-SY5Y cells, HepG2 cells, mouse colon tissue, rat colon tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue, human small intestine tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

The immunogen of 22335-1-AP is RCC1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RCC1 fusion protein Ag17809 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name regulator of chromosome condensation 1
Synonyms Cell cycle regulatory protein, CHC1, RCC1, RCC1 I
Calculated Molecular Weight 421 aa, 45 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC010067
Gene Symbol RCC1
Gene ID (NCBI) 1104
RRIDAB_10896498
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18754
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against full length RCC1 of human origin.

实验方案

Product Specific Protocols
WB protocol for RCC1 antibody 22335-1-APDownload protocol
IHC protocol for RCC1 antibody 22335-1-APDownload protocol
IF protocol for RCC1 antibody 22335-1-APDownload protocol
IP protocol for RCC1 antibody 22335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Pathol Oncol Res

RCC1 Expression as a Prognostic Marker in Colorectal Liver Oligometastases.

Authors - Yuxiang Deng
View Article