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CoraLite® Plus 488-conjugated RCAS1 Monoclonal antibody

RCAS1 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4H8A12

Cat No : CL488-66170

Print datasheet

Synonyms

EB9, EBAG9, PDAF, RCAS1

验证数据展示

  • IF Staining of MCF-7 using CL488-66170

    IF Staining of MCF-7 using CL488-66170

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 RCAS1 antibody (CL488-66170, Clone: 4H8A12 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-66170

    FC experiment of Jurkat using CL488-66170

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RCAS1 (CL488-66170, Clone:4H8A12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inMCF-7 cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy RCAS1 Ready-To-Use IHC Kit. RCAS1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66170-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66170 targets RCAS1 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human
Immunogen RCAS1 fusion protein Ag2905 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name estrogen receptor binding site associated, antigen, 9
Synonyms EB9, EBAG9, PDAF, RCAS1
Calculated Molecular Weight 213 aa, 24 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC017729
Gene Symbol EBAG9
Gene ID (NCBI) 9166
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00559
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Estrogen receptor-binding fragment-associated antigen 9 (EBAG9) gene was identified as an estrogen-responsive gene. The gene product, receptor-binding cancer antigen expressed on SiSo cells (RCAS1), is associated with aggressive characteristics and poor overall survival for 15 different human malignancies. The correlation between RCAS1 expression and several clinicopathological variables, including tumor size, clinical stage, invasion depth and lymph node metastasis highlights this molecule's clinical significance. Expression of RCAS1 in tumor cells plays an important role in evasion from host immune system resulting tumor progression, invasion and metastasis. Further exploration of RCAS1 biological function will facilitate development of novel therapeutic strategies that target RCAS1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RCAS1 antibody CL488-66170Download protocol
FC protocol for CL Plus 488 RCAS1 antibody CL488-66170Download protocol
Standard Protocols
Click here to view our Standard Protocols