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验证数据展示

  • WB analysis of HepG2 using 30044-1-AP (same clone as 30044-1-PBS)

    WB analysis of HepG2 using 30044-1-AP (same clone as 30044-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 30044-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30044-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 30044-1-AP (same clone as 30044-1-PBS)

    IP experiment of HepG2 using 30044-1-AP (same clone as 30044-1-PBS)

    IP result of anti-RBPJ (IP:30044-1-AP, 4ug; Detection:30044-1-AP 1:1000) with HepG2 cells lysate 1080 ug. This data was developed using the same antibody clone with 30044-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 30044-1-AP (same clone as 30044-1-PBS)

    IHC staining of human pancreas cancer using 30044-1-AP (same clone as 30044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30044-1-AP (RBPJ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30044-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBPJ antibody included

RBPJ Polyclonal antibody, PBS Only

RBPJ Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 30044-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RBPJ Ready-To-Use IHC Kit. RBPJ primary antibody included.

产品信息

30044-1-PBS targets RBPJ in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32552

Product name: Recombinant human RBPJ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 380-486 aa of BC064976

Sequence: MYRCGESMLCVVPDISAFREGWRWVRQPVQVPVTLVRNDGIIYSTSLTFTYTPEPGPRPHCSAAGAILRANSSQVPPNESNTNSEGSYTNASTNSTSVTSSTATVVS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 recombination signal binding protein for immunoglobulin kappa J region
别名
计算分子量 56 kDa
观测分子量~60 kDa
GenBank蛋白编号BC064976
基因名称 RBPJ
Gene ID (NCBI) 3516
RRIDAB_3086217
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ06330
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBPJ, also named as IGKJRB, IGKJRB1, RBPJK, RBPSUH, NY-REN-30, CBF-1 and CSL, belongs to the Su(H) family. It is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. RBPJ acts as a transcriptional repressor when it is not associated with Notch proteins. It is specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. RBPJ binds to the Runx3 promoter in the atrioventricular canal in vivo, and inhibition of Notch reduces RUNX3 expression in the cardiac cushion of embryonic hearts.

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