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验证数据展示

  • WB analysis using 84503-4-RR

    WB analysis using 84503-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84503-4-RR (RBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 84503-4-RR

    IHC staining of mouse liver using 84503-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84503-4-RR (RBP4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 84503-4-RR

    IHC staining of mouse liver using 84503-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84503-4-RR (RBP4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RBP4 Recombinant antibody

RBP4 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84503-4-RR

产品说明书

CloneNo. 242040D6

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

242040D6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart tissue, mouse lung tissue, mouse kidney tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84503-4-RR targets RBP4 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 retinol binding protein 4, plasma
别名 242040D6
计算分子量23kDa
观测分子量20-23 kDa
GenBank蛋白编号NM_011255.3
基因名称 Rbp4
Gene ID (NCBI) 19662
RRIDAB_3672014
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ00724
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)

实验方案

Product Specific Protocols
WB protocol for RBP4 antibody 84503-4-RRDownload protocol
IHC protocol for RBP4 antibody 84503-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols