验证数据展示
经过测试的应用
| Positive WB detected in | rat retina tissue, rabbit retina tissue, mouse retina tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60921-3-Ig targets RBP3 in WB, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat, rabbit |
| 免疫原 |
CatNo: Ag6182 Product name: Recombinant human RBP3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 898-1247 aa of BC039844 Sequence: AMSATTGKAWDLAGVEPDITVPMSEALSIAQDIVALRAKVPTVLQTAGKLVADNYASAELGAKMATKLSGLQSRYSRVTSEVALAEILGADLQMLSGDPHLKAAHIPENAKDRIPGIVPMQIPSPEVFEELIKFSFHTNVLEDNIGYLRFDMFGDGELLTQVSRLLVEHIWKKIMHTDAMIIDMRFNIGGPTSSIPILCSYFFDEGPPVLLDKIYSRPDDSVSELWTHAQVVGERYGSKKSMVILTSSVTAGTAEEFTYIMKRLGRALVIGEVTSGGCQPPQTYHVDDTNLYLTIPTARSVGASDGSSWEGVGVTPHVVVPAEEALARAKEMLQHNQLRVKRSPGLQDHL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | retinol binding protein 3, interstitial |
| 别名 | Interphotoreceptor retinoid-binding protein, Interstitial retinol-binding protein, Retinol binding protein 3, Retinol-binding protein 3 |
| 计算分子量 | 135 kDa |
| 观测分子量 | 135 kDa |
| GenBank蛋白编号 | BC039844 |
| 基因名称 | RBP3 |
| Gene ID (NCBI) | 5949 |
| RRID | AB_3743243 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P10745 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBP3 (retinoid-binding protein 3), also called interstitial retinoid-binding protein (IRBP), is a large glycoprotein that is synthesized and secreted by rod photoreceptor cells into the interphotoreceptor matrix. It is known to bind retinoids and fatty acids. RBP3 migrates around 136-140 kDa in the western blot analysis. (PMID: 15928609)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RBP3 antibody 60921-3-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
