验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, C6 cells |
Positive IP detected in | HeLa cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11061-2-AP targets RBMS1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, RIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human |
免疫原 | RBMS1 fusion protein Ag1493 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | RNA binding motif, single stranded interacting protein 1 |
别名 | MSSP 3, MSSP 2, MSSP 1, MSSP, HCC 4 |
计算分子量 | 45 kDa |
观测分子量 | 45 kDa |
GenBank蛋白编号 | BC018951 |
基因名称 | RBMS1 |
Gene ID (NCBI) | 5937 |
RRID | AB_2179121 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P29558 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for RBMS1 antibody 11061-2-AP | Download protocol |
IF protocol for RBMS1 antibody 11061-2-AP | Download protocol |
IP protocol for RBMS1 antibody 11061-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Epigenetics Suppressing circIDE/miR-19b-3p/RBMS1 axis exhibits promoting-tumour activity through upregulating GPX4 to diminish ferroptosis in hepatocellular carcinoma | ||
Mol Cell Biol Increased ANKRD1 Levels in Early Senescence Mediated by RBMS1-Elicited ANKRD1 mRNA Stabilization |