• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RBMS1 Polyclonal antibody

RBMS1 Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11061-2-AP

Print datasheet

Synonyms

C2orf12, HCC 4, MSSP, MSSP 1, MSSP 2, MSSP 3, MSSP1, RBMS1, SCR2, YC1

验证数据展示

  • WB analysis using 11061-2-AP

    WB analysis using 11061-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11061-2-AP (RBMS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 11061-2-AP

    IF Staining of HepG2 using 11061-2-AP

    Immunofluorescent analysis of HepG2 cells, using RBMS1 antibody 11061-2-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of HeLa using 11061-2-AP

    IP experiment of HeLa using 11061-2-AP

    IP result of anti-RBMS1 (IP:11061-2-AP, 4ug; Detection:11061-2-AP 1:500) with HeLa cells lysate 1200ug.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, C6 cells
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11061-2-AP targets RBMS1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RBMS1 fusion protein Ag1493 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RNA binding motif, single stranded interacting protein 1
Synonyms C2orf12, HCC 4, MSSP, MSSP 1, MSSP 2, MSSP 3, MSSP1, RBMS1, SCR2, YC1
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC018951
Gene Symbol RBMS1
Gene ID (NCBI) 5937
RRIDAB_2179121
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29558
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RBMS1 antibody 11061-2-APDownload protocol
IF protocol for RBMS1 antibody 11061-2-APDownload protocol
IP protocol for RBMS1 antibody 11061-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Epigenetics

Suppressing circIDE/miR-19b-3p/RBMS1 axis exhibits promoting-tumour activity through upregulating GPX4 to diminish ferroptosis in hepatocellular carcinoma

Authors - Hang Zhai
View Article