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验证数据展示

  • WB analysis using 11061-2-AP

    WB analysis using 11061-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11061-2-AP (RBMS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 11061-2-AP

    IF Staining of HepG2 using 11061-2-AP

    Immunofluorescent analysis of HepG2 cells, using RBMS1 antibody 11061-2-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of HeLa using 11061-2-AP

    IP experiment of HeLa using 11061-2-AP

    IP result of anti-RBMS1 (IP:11061-2-AP, 4ug; Detection:11061-2-AP 1:500) with HeLa cells lysate 1200ug.

RBMS1 Polyclonal antibody

RBMS1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 11061-2-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA and More (2)

别名

MSSP 3, MSSP 2, MSSP 1, MSSP, HCC 4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, C6 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11061-2-AP targets RBMS1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, RIP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag1493

Product name: Recombinant human RBMS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-406 aa of BC018951

Sequence: MGKVWKQQMYPQYATYYYPQYLQAKQSLVPAHPMAPPSPSTTSSNNNSSSSSNSGWDQLSKTNLYIRGLPPHTTDQDLVKLCQPYGKIVSTKAILDKTTNKCKGYGFVDFDSPAAAQKAVSALKASGVQAQMAKQQEQDPTNLYISNLPLSMDEQELENMLKPFGQVISTRILRDSSGTSRGVGFARMESTEKCEAVIGHFNGKFIKTPPGVSAPTEPLLCKFADGGQKKRQNPNKYIPNGRPWHREGEVRLAGMTLTYDPTTAAIQNGFYPSPYSIATNRMITQTSITPYIASPVSAYQVQSPSWMQPQPYILQHPGAVLTPSMEHTMSLQPASMISPLAQQMSHLSLGSTGTYMPATSAMQGAYLPQYAHMQTTAVPVEEASGQQQVAVETSNDHSPYTFQPNK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif, single stranded interacting protein 1
别名 MSSP 3, MSSP 2, MSSP 1, MSSP, HCC 4
计算分子量 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC018951
基因名称 RBMS1
Gene ID (NCBI) 5937
RRIDAB_2179121
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP29558
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for RBMS1 antibody 11061-2-APDownload protocol
IP protocol for RBMS1 antibody 11061-2-APDownload protocol
WB protocol for RBMS1 antibody 11061-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Epigenetics

Suppressing circIDE/miR-19b-3p/RBMS1 axis exhibits promoting-tumour activity through upregulating GPX4 to diminish ferroptosis in hepatocellular carcinoma

Authors - Hang Zhai
View Article
humanRIP

Mol Cell Biol

Increased ANKRD1 Levels in Early Senescence Mediated by RBMS1-Elicited ANKRD1 mRNA Stabilization

Authors - Chang Hoon Shin
View Article
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