验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells |
| Positive IHC detected in | human stomach tissue, human lung tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
19930-1-AP targets RBM5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | RBM5 fusion protein Ag13908 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RNA binding motif protein 5 |
| 别名 | RMB5, Renal carcinoma antigen NY-REN-9, Protein G15, LUCA15, H37 |
| 计算分子量 | 815 aa, 92 kDa |
| 观测分子量 | 110-115 kDa |
| GenBank蛋白编号 | BC002957 |
| 基因名称 | RBM5 |
| Gene ID (NCBI) | 10181 |
| RRID | AB_2878625 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P52756 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBM5, also named as RNA-binding protein 5, is a 815 amino acid protein, which contains 2 RRM (RNA recognition motif) domains and Belongs to the RBM5/RBM10 family. RBM5 localizes in the nucleus and is a component of the spliceosome A complex. RBM5 regulates alternative splicing of a number of mRNAs and may modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. RBM5 may both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. The phosphorylation of RBM5 protein would influence the detected molecular weight. A 105-110 KDa band was also reported (PMID: 27957556).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RBM5 antibody 19930-1-AP | Download protocol |
| IHC protocol for RBM5 antibody 19930-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem CHIP suppresses the proliferation and migration of A549 cells by mediating the ubiquitination of eIF2α and upregulation of tumor suppressor RBM5 | ||
J Neurotrauma RNA Binding Motif 5 Gene Deletion Modulates Cell Signaling in a Sex-Dependent Manner but not Hippocampal Cell Death. | ||
Cell Death Discov Neuronal RBM5 modulates cell signaling responses to traumatic and hypoxic-ischemic injury in a sex-dependent manner |
