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验证数据展示

  • WB analysis of HEK-293 using 15412-1-AP

    WB analysis of HEK-293 using 15412-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15412-1-AP (RBM4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 15412-1-AP

    IP experiment of HEK-293 using 15412-1-AP

    IP result of anti-RBM4B (IP:15412-1-AP, 3ug; Detection:15412-1-AP 1:300) with HEK-293 cells lysate 1000ug.

RBM4B Polyclonal antibody

RBM4B Polyclonal Antibody for WB, IP, ELISA
Cat No. 15412-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

RBM30, RBM4B, RBM4L, RNA binding motif protein 30, RNA binding motif protein 4B, RNA binding protein 30, RNA binding protein 4B, ZCCHC15, ZCRB3B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15412-1-AP targets RBM4B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7679

Product name: Recombinant human RBM4B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-359 aa of BC003503

Sequence: MVKLFIGNLPREATEQEIRSLFEQYGKVLECDIIKNYGFVHIEDKTAAEDAIRNLHHYKLHGVNINVEASKNKSKASTKLHVGNISPTCTNQELRAKFEEYGPVIECDIVKDYAFVHMERAEDAVEAIRGLDNTEFQGKRMHVQLSTSRLRTAPGMGDQSGCYRCGKEGHWSKECPVDRTGRVADFTEQYNEQYGAVRTPYTMGYGESMYYNDAYGALDYYKRYRVRSYEAVAAAAAASAYNYAEQTMSHLPQVQSTTVTSHLNSTSVDPYDRHLLPNSGAAATSAAMAAAAATTSSYYGRDRSPLRRAAAMLPTVGEGYGYGPESELSQASAATRNSLYDMARYEREQYVDRARYSAF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 4B
别名 RBM30, RBM4B, RBM4L, RNA binding motif protein 30, RNA binding motif protein 4B, RNA binding protein 30, RNA binding protein 4B, ZCCHC15, ZCRB3B
计算分子量 40 kDa
观测分子量 36-40 kDa
GenBank蛋白编号BC003503
基因名称 RBM4B
Gene ID (NCBI) 83759
RRIDAB_2181210
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQ04
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IP protocol for RBM4B antibody 15412-1-APDownload protocol
WB protocol for RBM4B antibody 15412-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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