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验证数据展示

  • WB analysis using 67420-1-Ig

    WB analysis using 67420-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67420-1-Ig (RBM39 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67420-1-Ig

    IHC staining of human breast cancer using 67420-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67420-1-Ig (RBM39 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RBM39 antibody included
  • IF Staining of HepG2 using 67420-1-Ig

    IF Staining of HepG2 using 67420-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67420-1-Ig (RBM39 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 67420-1-Ig

    WB analysis using 67420-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67420-1-Ig (RBM39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67420-1-Ig

    IF Staining of HepG2 using 67420-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBM39 antibody (67420-1-Ig, Clone: 2D2C8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

RBM39 Monoclonal antibody

RBM39 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67420-1-Ig

产品说明书

CloneNo. 2D2C8

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CAPER, CAPERalpha, CC1.3, DKFZp781C0423, FLJ44170, fSAP59, HCC1, RBM39, RNA binding motif protein 39, RNA binding protein 39, RNPC2, Splicing factor HCC1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells, HeLa cells, LNCaP cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, Neuro-2a cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RBM39 Ready-To-Use IHC Kit. RBM39 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67420-1-Ig targets RBM39 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 RBM39 fusion protein Ag15766 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 RNA binding motif protein 39
别名 CAPER, CAPERalpha, CC1.3, DKFZp781C0423, FLJ44170, fSAP59, HCC1, RBM39, RNA binding motif protein 39, RNA binding protein 39, RNPC2, Splicing factor HCC1
计算分子量 530 aa, 59 kDa
观测分子量 60-66 kDa
GenBank蛋白编号BC141835
基因名称 RBM39
Gene ID (NCBI) 9584
RRIDAB_2882660
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14498
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM39, also named as HCC1 or RNPC2, is a 530 amino acid protein, which contains three RRM (RNA recognition motif) domains and belongs to the splicing factor SR family. RBM39 is widely expressed in various tissues and with highly expression in pancreas, skeletal muscle, lung and brain. RBM39 is a transcriptional coactivator for steroid nuclear receptors ESR1/ER-alpha and ESR2/ER-beta, and JUN/AP-1. RBM39 may be involved in pre-mRNA splicing process.

实验方案

Product Specific Protocols
WB protocol for RBM39 antibody 67420-1-IgDownload protocol
IHC protocol for RBM39 antibody 67420-1-IgDownload protocol
IF protocol for RBM39 antibody 67420-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols