验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HeLa cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32834-1-AP targets RBM34 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag37711 Product name: Recombinant human RBM34 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 77-425 aa of BC029451 Sequence: TKRNEEEESTSQIERPLSQEPAKKVKAKKKHTNAEKKLADRESALASADLEEEIHQKQGQKRKNSQPGVKVADRKILDDTEDTVVSQRKKIQINQEEERLKNERTVFVGNLPVTCNKKKLKSFFKEYGQIESVRFRSLIPAEGTLSKKLAAIKRKIHPDQKNINAYVVFKEESAATQALKRNGAQIADGFRIRVDLASETSSRDKRSVFVGNLPYKVEESAIEKHFLDCGSIMAVRIVRDKMTGIGKGFGYVLFENTDSVHLALKLNNSELMGRKLRVMRSVNKEKFKQQNSNPRLKNVSKPKQGLNFTSKTAEGHPKSLFIGEKAVLLKTKKKGQKKSGRPKKQRKQK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RNA binding motif protein 34 |
| 别名 | KIAA0117, RNA-binding motif protein 34, RNA-binding protein 34 |
| 计算分子量 | 430 aa, 49 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC029451 |
| 基因名称 | RBM34 |
| Gene ID (NCBI) | 23029 |
| RRID | AB_3742756 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P42696 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBM34 (RNA Binding Motif Protein 34) belongs to the RNA-binding motif protein (RBM) family, whose members all contain an RNA recognition motif (RRM) that enables specific binding to RNA molecules and participation in multiple aspects of RNA metabolism. RBM34 is primarily localized in the nucleus and functionally acts as an RNA-binding protein that plays an important role in pre-mRNA splicing regulation-it modulates alternative splicing events through interactions with splicing factors, thereby influencing mRNA maturation and diversity.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RBM34 antibody 32834-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
