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验证数据展示

  • WB analysis using 31850-1-AP (same clone as 31850-1-PBS)

    WB analysis using 31850-1-AP (same clone as 31850-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31850-1-AP (RBM27 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31850-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 31850-1-AP (same clone as 31850-1-PBS)

    WB analysis of HeLa using 31850-1-AP (same clone as 31850-1-PBS)

    WB result of RBM27 antibody (31850-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM27 transfected HeLa cells. This data was developed using the same antibody clone with 31850-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 31850-1-AP (same clone as 31850-1-PBS)

    IF Staining of U2OS using 31850-1-AP (same clone as 31850-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RBM27 antibody (31850-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 31850-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RBM27 Polyclonal antibody, PBS Only

RBM27 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 31850-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

KIAA1311, RNA Binding Motif Protein 27, RNA-Binding Protein 27

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31850-1-PBS targets RBM27 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35822

Product name: Recombinant human RBM27 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 680-815 aa of NM_018989

Sequence: TLQSSAQLLLQQQQTLSHLSQQHHHLPQHLHQQQVLVAQSAPSTVHGGIQKMMSKPQTSGAYVLNKVPVKHRLGHAGGNQSDASHLLNQSGGAGEDCQIFSTPGHPKMIYSSSNLKTPSKLCSGSKSHDVQEVLKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 27
别名 KIAA1311, RNA Binding Motif Protein 27, RNA-Binding Protein 27
计算分子量119 kDa
观测分子量140 kDa
GenBank蛋白编号NM_018989
基因名称 RBM27
Gene ID (NCBI) 54439
RRIDAB_3670129
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9P2N5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBM27 has been identified as a candidate autism gene, and is present in cytoplasmic and and nuclear speckles. RBM27 encodes a protein containing an RNA-Binding Motif, which confers its ability to bind RNA. Therefore, RBM27 is important in mRNA processing and the turnover of nuclear polyadenylate (pA+) RNA.

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