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验证数据展示

  • WB analysis using 16823-1-AP

    WB analysis using 16823-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16823-1-AP (RBM26 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 16823-1-AP

    IHC staining of human brain using 16823-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16823-1-AP (RBM26 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 16823-1-AP

    IHC staining of human brain using 16823-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16823-1-AP (RBM26 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 16823-1-AP

    IF Staining of HepG2 using 16823-1-AP

    Immunofluorescent analysis of HepG2 cells, using RBM26 antibody 16823-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of HEK-293 using 16823-1-AP

    IP experiment of HEK-293 using 16823-1-AP

    IP result of anti-RBM26 (IP:16823-1-AP, 4ug; Detection:16823-1-AP 1:5000) with HEK-293 cells lysate 1400 ug.

RBM26 Polyclonal antibody

RBM26 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16823-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

RNA-binding motif protein 26, RBM 26, PRO1777, CTCL tumor antigen se70-2, C13orf10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16823-1-AP targets RBM26 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 RBM26 fusion protein Ag10398 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 26
别名 RNA-binding motif protein 26, RBM 26, PRO1777, CTCL tumor antigen se70-2, C13orf10
计算分子量 983 aa, 111 kDa
观测分子量 125 kDa
GenBank蛋白编号BC000791
基因名称 RBM26
Gene ID (NCBI) 64062
RRIDAB_2175942
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T8P6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RBM26 antibody 16823-1-APDownload protocol
IHC protocol for RBM26 antibody 16823-1-APDownload protocol
IF protocol for RBM26 antibody 16823-1-APDownload protocol
IP protocol for RBM26 antibody 16823-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Dual modes of ZFC3H1 confer selectivity in nuclear RNA sorting

Authors - Jing Fan
View Article