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验证数据展示

  • IHC staining of rat heart using 31177-1-AP

    IHC staining of rat heart using 31177-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 31177-1-AP (RBM20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 31177-1-AP

    IHC staining of mouse heart using 31177-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31177-1-AP (RBM20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RBM20 Polyclonal antibody

RBM20 Polyclonal Antibody for IHC, ELISA
Cat No. 31177-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, ELISA

别名

RNA binding motif protein 20, RNA binding protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inrat heart tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31177-1-AP targets RBM20 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34322

Product name: Recombinant human RBM20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 667-840 aa of NM_001134363

Sequence: ADWGNGRDSWEHSPYARREEERDPAPWRDNGDDKRDRMDPWAHDRKHHPRQLDKAELDERPEGGRPHREKYPRSGSPNLPHSVSSYKSREDGYYRKEPKAKSDKYLKQQQDAPGRSRRKDEARLRESRHPHPDDSGKEDGLGPKVTRAPEGAKAKQNEKNKTKRTDRDQEGADD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 20
别名 RNA binding motif protein 20, RNA binding protein
计算分子量134 kDa
GenBank蛋白编号NM_001134363
基因名称 RBM20
Gene ID (NCBI) 282996
RRIDAB_3669884
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ5T481
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM20 (RNA binding motif protein 20) is a striated muscle-specific splicing factor that regulates alternative splicing of many cardiac genes encoding sarcomere and calcium-regulatory proteins (PMID: 36417486). RBM20 has been identified as a crucial RNA-binding protein that controls the splicing of TTN (PMID: 30547036). RBM20 is expressed in all striated muscles but is highest in cardiac tissue (PMID: 34575212).

实验方案

Product Specific Protocols
IHC protocol for RBM20 antibody 31177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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