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验证数据展示

  • WB analysis of mouse colon using 19095-1-AP

    WB analysis of mouse colon using 19095-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 19095-1-AP

    IHC staining of human colon using 19095-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 19095-1-AP (RBM19 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 19095-1-AP

    IHC staining of human colon using 19095-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 19095-1-AP (RBM19 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of MCF-7 using 19095-1-AP

    IF Staining of MCF-7 using 19095-1-AP

    Immunofluorescent analysis of MCF-7 cells, using RBM19 antibody 19095-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

RBM19 Polyclonal antibody

RBM19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19095-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

KIAA0682, RBM19, RNA binding motif protein 19

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse colon tissue, COLO 320 cells, mouse small intestine tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19095-1-AP targets RBM19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13545

Product name: Recombinant human RBM19 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC006137

Sequence: MSRLIVKNLPNGMKEERFRQLFAAFGTLTDCSLKFTKDGKFRKFGFIGFKSEEEAQKAQKHFNKSFIDTSRITVEFCKSFGDPAKPRAWSKHAQKPSQPKQPPKDSTTPEIKKDEKKKKVAGQLEKLKEDTEFQEFLSVHQRRAQAATWANDGLDAEPSKGKSKPASDYLNFDSDSGQESEEEGAGEDLEEEASLEPKAAVQKELSDMDYLKSKMVKAGSSSSSEEEESEDEAVHCDEGSEAEEEDSSATPVLQERDSRGAGQEQGMPAGKKRPPEARAETEKPANQKEPTTCHTVKLRG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 19
别名 KIAA0682, RBM19, RNA binding motif protein 19
计算分子量 107 kDa
观测分子量 120 kDa
GenBank蛋白编号BC006137
基因名称 RBM19
Gene ID (NCBI) 9904
RRIDAB_10640906
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y4C8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM19, also named as KIAA0682, is a 960 amino acid protein, which belongs to the RRM MRD1 family. RBM19 is expressed in the crypts of Lieberkuhn of the intestine and in intestinal neoplasia. RBM19 plays a role in embryo pre-implantation development. The calculated molecular weight of RBM19 is 107 kDa, but the modified RBM19 is about 120 kDa.

实验方案

Product Specific Protocols
IF protocol for RBM19 antibody 19095-1-APDownload protocol
IHC protocol for RBM19 antibody 19095-1-APDownload protocol
WB protocol for RBM19 antibody 19095-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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