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RBM17 Polyclonal antibody

RBM17 Polyclonal Antibody for WB,IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,IP,ELISA

Conjugate

Unconjugated

Cat No : 15374-1-AP

Print datasheet

Synonyms

45 kDa splicing factor, RBM17, RNA binding motif protein 17, SPF45, Splicing factor 45

验证数据展示

  • WB analysis using 15374-1-AP

    WB analysis using 15374-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15374-1-AP (RBM17 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 15374-1-AP

    IP experiment of HeLa using 15374-1-AP

    IP result of anti-RBM17 (IP:15374-1-AP, 4ug; Detection:15374-1-AP 1:500) with HeLa cells lysate 2600ug.

经过测试的应用

Positive WB detected inHeLa cells, mouse thymus tissue, rat thymus tissue
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15374-1-AP targets RBM17 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,IP,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen RBM17 fusion protein Ag0985 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RNA binding motif protein 17
Synonyms 45 kDa splicing factor, RBM17, RNA binding motif protein 17, SPF45, Splicing factor 45
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC007871
Gene Symbol RBM17
Gene ID (NCBI) 84991
RRIDAB_2175914
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96I25
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.

实验方案

Product Specific Protocols
WB protocol for RBM17 antibody 15374-1-APDownload protocol
IP protocol for RBM17 antibody 15374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Hum Mol Genet

Interactome network analysis identifies multiple caspase-6 interactors involved in the pathogenesis of HD.

Authors - Sean-Patrick Riechers
View Article