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验证数据展示

  • WB analysis using 30990-1-AP

    WB analysis using 30990-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30990-1-AP (RBM12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 30990-1-AP

    IF Staining of U2OS using 30990-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RBM12 antibody (30990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human tonsillitis using 30990-1-AP

    IHC staining of human tonsillitis using 30990-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 30990-1-AP (RBM12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RBM12 Polyclonal antibody

RBM12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30990-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

RNA-binding protein 12, RNA-binding motif protein 12, RNA binding protein 12, RNA binding motif protein 12, KIAA0765

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMOLT-4 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30990-1-AP targets RBM12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34621

Product name: Recombinant human RBM12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 834-932 aa of BC013981

Sequence: GPGPIHIGGPPGFASSSGKPGPTVIKVQNMPFTVSIDEILDFFYGYQVIPGSVCLKYNEKGMPTGEAMVAFESRDEATAAVIDLNDRPIGSRKVKLVLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 12
别名 RNA-binding protein 12, RNA-binding motif protein 12, RNA binding protein 12, RNA binding motif protein 12, KIAA0765
观测分子量100 kDa
GenBank蛋白编号BC013981
基因名称 RBM12
Gene ID (NCBI) 10137
RRIDAB_3669810
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9NTZ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA binding motif protein 12 (RBM12) is a member of the RBM family. RBM proteins are involved in RNA metabolism, including splicing, transport, translation and stability. RBM12 has been identified as a key protein that increases the expression of PD-L1, thereby promoting immune evasion in hepatocellular carcinoma (PMID: 39187545).

实验方案

Product Specific Protocols
IF protocol for RBM12 antibody 30990-1-APDownload protocol
IHC protocol for RBM12 antibody 30990-1-APDownload protocol
WB protocol for RBM12 antibody 30990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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