验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31590-1-AP targets RBKS in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | RBKS fusion protein Ag36374 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribokinase |
| 别名 | RK, ribokinase, RBSK |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC017425 |
| 基因名称 | RBKS |
| Gene ID (NCBI) | 64080 |
| RRID | AB_3670048 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9H477 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBKS also known as ribokinase, is a member of the carbohydrate kinase PfkB family. The protein encoded by this gene phosphorylates ribose to form ribose-5-phosphate in the presence of ATP and magnesium, which is the first step in ribose metabolism. This process is crucial for the metabolism of ribose, a sugar that is a component of RNA and ATP. In summary, RBKS is an important gene involved in the initial step of ribose metabolism, with its protein product playing a key role in phosphorylating ribose to ribose-5-phosphate, a reaction that is essential for further metabolic processes involving this sugar.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RBKS antibody 31590-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |