验证数据展示
经过测试的应用
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26367-1-AP targets RBCK1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag24405 Product name: Recombinant human RBCK1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 225-500 aa of BC015219 Sequence: EERARLAGEEEALRQYQQRKQQQQEGNYLQHVQLDQRSLVLNTEPAECPVCYSVLAPGEAVVLRECLHTFCRECLQGTIRNSQEAEVSCPFIDNTYSCSGKLLEREIKALLTPEDYQRFLDLGISIAENRSAFSYHCKTPDCKGWCFFEDDVNEFTCPVCFHVNCLLCKAIHEQMNCKEYQEDLALRAQNDVAARQTTEMLKVMLQQGEAMRCPQCQIVVQKKDGCDWIRCTVCHTEICWVTKGPRWGPGGPGDTSGGCRCRVNGIPCHPSCQNCH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RanBP-type and C3HC4-type zinc finger containing 1 |
| 别名 | C20orf18, HBV associated factor 4, HOIL 1, HOIL1, RBCK1, RBCK2, RING finger protein 54, RNF54, UBCE7IP3, XAP3, XAP4, ZRANB4 |
| 计算分子量 | 58 kDa |
| 观测分子量 | 57 kDa |
| GenBank蛋白编号 | BC015219 |
| 基因名称 | RBCK1 |
| Gene ID (NCBI) | 10616 |
| RRID | AB_2880493 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BYM8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for RBCK1 antibody 26367-1-AP | Download protocol |
| IP protocol for RBCK1 antibody 26367-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis RBCK1 regulates the progression of ER-positive breast cancer through the HIF1α signaling | ||
Oncogene The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis
| ||
Front Immunol Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma | ||
Hum Cell Regulation of PTEN and ovarian cancer progression by an E3 ubiquitin ligase RBCK1.
| ||
Biochem Biophys Res Commun Mechanistic insights into the homo-dimerization of HOIL-1L and SHARPIN | ||
BMC Musculoskelet Disord Polyglucosan body myopathy 1 may cause cognitive impairment: a case report from China. |
