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RBBP9 Monoclonal antibody

RBBP9 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H6D8

Cat No : 66015-1-Ig

Print datasheet

Synonyms

RBBP9

验证数据展示

  • WB analysis of A549 using 66015-1-Ig

    WB analysis of A549 using 66015-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66015-1-Ig (RBBP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 66015-1-Ig

    IF Staining of HeLa using 66015-1-Ig

    Immunofluorescent analysis of Hela cells, using RBBP9 antibody 66015-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

  • IHC staining of human pancreas cancer using 66015-1-Ig

    IHC staining of human pancreas cancer using 66015-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-1-Ig (RBBP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 66015-1-Ig

    IHC staining of human pancreas cancer using 66015-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-1-Ig (RBBP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inhuman pancreas cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 66015-1-Ig is RBBP9 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human
Immunogen RBBP9 fusion protein Ag17864 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name retinoblastoma binding protein 9
Synonyms RBBP9
Calculated Molecular Weight 186 aa, 21 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC015938
Gene Symbol RBBP9
Gene ID (NCBI) 10741
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75884
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell–cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID:20080647)

实验方案

Product Specific Protocols
WB protocol for RBBP9 antibody 66015-1-IgDownload protocol
IHC protocol for RBBP9 antibody 66015-1-IgDownload protocol
IF protocol for RBBP9 antibody 66015-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols