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验证数据展示

  • WB analysis using 84330-1-RR

    WB analysis using 84330-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84330-1-RR (RBBP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 84330-1-RR

    IF Staining of A431 using 84330-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBBP7 antibody (84330-1-RR, Clone: 241189D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 84330-1-RR

    FC experiment of HeLa using 84330-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RBBP7 Recombinant antibody (84330-1-RR, Clone:241189D6) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

  • IHC staining of human placenta using 84330-1-RR

    IHC staining of human placenta using 84330-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RBBP7 antibody included
  • IHC staining of human placenta using 84330-1-RR

    IHC staining of human placenta using 84330-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RBBP7 antibody included
  • IHC staining of human rectal cancer using 84330-1-RR

    IHC staining of human rectal cancer using 84330-1-RR

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RBBP7 antibody included
  • IHC staining of human rectal cancer using 84330-1-RR

    IHC staining of human rectal cancer using 84330-1-RR

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RBBP7 antibody included

RbAp46 Recombinant antibody

RbAp46 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84330-1-RR

产品说明书

CloneNo. 241189D6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

RBBP7, Retinoblastoma-binding protein 7, RBBP-7, Nucleosome-remodeling factor subunit RBAP46, Histone-binding protein RBBP7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HEK-293 cells, Jurkat cells, MCF-7 cells, SH-SY5Y cells
Positive IHC detected inhuman rectal cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RBBP7 Ready-To-Use IHC Kit. RBBP7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84330-1-RR targets RbAp46 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 retinoblastoma binding protein 7
别名 RBBP7, Retinoblastoma-binding protein 7, RBBP-7, Nucleosome-remodeling factor subunit RBAP46, Histone-binding protein RBBP7
计算分子量 48 kDa
GenBank蛋白编号NM_002893
基因名称 RBBP7
Gene ID (NCBI) 5931
RRIDAB_3671871
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ16576
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBBP7, also named as RBAP46, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP7 is a core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP7.

实验方案

Product Specific Protocols
WB protocol for RbAp46 antibody 84330-1-RRDownload protocol
IHC protocol for RbAp46 antibody 84330-1-RRDownload protocol
IF protocol for RbAp46 antibody 84330-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols