RB1 Polyclonal antibody

RB1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 25628-1-AP

Print datasheet

Synonyms

OSRC, p105 Rb, pp110, pRb, RB, RB1, retinoblastoma 1



经过测试的应用

Positive WB detected inJurkat cells, A431 cells
Positive IP detected inJurkat cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

产品信息

The immunogen of 25628-1-AP is RB1 Fusion Protein expressed in E. coli.

Tested Applications IF, IP, WB, ELISA
Cited ApplicationsIF, WB
Tested Reactivity human
Cited Reactivityhuman
Immunogen RB1 fusion protein Ag22578 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name retinoblastoma 1
Synonyms OSRC, p105 Rb, pp110, pRb, RB, RB1, retinoblastoma 1
Calculated molecular weight 928 aa, 106 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC039060
Gene symbol RB1
UniprotP06400
Gene ID (NCBI) 5925
RRIDAB_2880168
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RB1 antibody 25628-1-APDownload protocol
IF protocol for RB1 antibody 25628-1-APDownload protocol
IP protocol for RB1 antibody 25628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Artif Cells Nanomed Biotechnol

Ribociclib (LEE011) suppresses cell proliferation and induces apoptosis of MDA-MB-231 by inhibiting CDK4/6-cyclin D-Rb-E2F pathway.

Authors - Tianqi Li
humanWB

Oncol Rep

Actin-capping protein CapG is associated with prognosis, proliferation and metastasis in human glioma.

Authors - Da-Peng Yun
humanIF

Oncol Lett

14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells.

Authors - Kun Lu
humanWB

Cell Death Dis

MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis.

Authors - Fengxia Chen
humanWB

Biomed Pharmacother

Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo.

Authors - Yudi Xiong