验证数据展示
经过测试的应用
Positive WB detected in | HL-60 cells, HepG2 cells, PC-12 cells |
Positive IHC detected in | human lung cancer tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21753-1-AP targets RASSF7 in WB, IHC, ELISA applications and shows reactivity with human, Rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human, Rat |
免疫原 | RASSF7 fusion protein Ag16123 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | Ras association (RalGDS/AF-6) domain family (N-terminal) member 7 |
别名 | C11orf13, HRAS1, HRC1, RASSF7 |
计算分子量 | 373 aa, 40 kDa |
观测分子量 | 40 kDa |
GenBank蛋白编号 | BC106921 |
基因名称 | RASSF7 |
Gene ID (NCBI) | 8045 |
RRID | AB_10858387 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q02833 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RASSF7, previously known as HRC1 (HRAS1 cluster 1) and C11orf13, is a member of the N-terminal RASSF family. RASSF7 protein localises to the centrosome and plays a key role in mitosis. RASSF7 is expressed in a broad range of different cell types and that this expression could be enhanced following exposure to hypoxia. RASSF7 has three isoforms with the MW 40 kDa, 36 kDa and 35 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for RASSF7 antibody 21753-1-AP | Download protocol |
IHC protocol for RASSF7 antibody 21753-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |