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验证数据展示

  • WB analysis using 21753-1-AP

    WB analysis using 21753-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21753-1-AP (RASSF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 21753-1-AP

    IHC staining of human lung cancer using 21753-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21753-1-AP (RASSF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 21753-1-AP

    IHC staining of human lung cancer using 21753-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21753-1-AP (RASSF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RASSF7 Polyclonal antibody

RASSF7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21753-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, Rat

应用

WB, IHC, ELISA

别名

C11orf13, HRAS1, HRC1, RASSF7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, HepG2 cells, PC-12 cells
Positive IHC detected inhuman lung cancer tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21753-1-AP targets RASSF7 in WB, IHC, ELISA applications and shows reactivity with human, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, Rat
免疫原 RASSF7 fusion protein Ag16123 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Ras association (RalGDS/AF-6) domain family (N-terminal) member 7
别名 C11orf13, HRAS1, HRC1, RASSF7
计算分子量 373 aa, 40 kDa
观测分子量 40 kDa
GenBank蛋白编号BC106921
基因名称 RASSF7
Gene ID (NCBI) 8045
RRIDAB_10858387
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02833
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RASSF7, previously known as HRC1 (HRAS1 cluster 1) and C11orf13, is a member of the N-terminal RASSF family. RASSF7 protein localises to the centrosome and plays a key role in mitosis. RASSF7 is expressed in a broad range of different cell types and that this expression could be enhanced following exposure to hypoxia. RASSF7 has three isoforms with the MW 40 kDa, 36 kDa and 35 kDa.

实验方案

Product Specific Protocols
WB protocol for RASSF7 antibody 21753-1-APDownload protocol
IHC protocol for RASSF7 antibody 21753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols