验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, COLO 320 cells, HeLa cells, HepG2 cells, MDA-MB-453s cells, PC-3 cells, U-937 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human ovary tumor tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11921-1-AP targets RASSF6 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, CoIP, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | RASSF6 fusion protein Ag2565 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Ras association (RalGDS/AF-6) domain family member 6 |
| 别名 | Ras association domain-containing protein 6 |
| 计算分子量 | 369 aa, 43 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | BC058835 |
| 基因名称 | RASSF6 |
| Gene ID (NCBI) | 166824 |
| RRID | AB_874225 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6ZTQ3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RASSF6 antibody 11921-1-AP | Download protocol |
| IHC protocol for RASSF6 antibody 11921-1-AP | Download protocol |
| IF protocol for RASSF6 antibody 11921-1-AP | Download protocol |
| IP protocol for RASSF6 antibody 11921-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget RASSF6 downregulation promotes the epithelial-mesenchymal transition and predicts poor prognosis in colorectal cancer. | ||
Mol Cancer The novel RASSF6 and RASSF10 candidate tumour suppressor genes are frequently epigenetically inactivated in childhood leukaemias. | ||
Epigenetics RASSF6 exhibits promoter hypermethylation in metastatic melanoma and inhibits invasion in melanoma cells. | ||
J Genet Genomics RASSF6-TRIM16 axis promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma.
| ||
J Cell Physiol MiR-496 promotes migration and epithelial-mesenchymal transition by targeting RASSF6 in colorectal cancer. | ||
Ann Surg Oncol Decreased expression of RASSF6 is a novel independent prognostic marker of a worse outcome in gastric cancer patients after curative surgery. |
