验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, NCI-H1299 cells, U2OS cells, MG-63 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 2 publications below |
产品信息
19118-1-AP targets RASSF4 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | RASSF4 fusion protein Ag5780 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Ras association (RalGDS/AF-6) domain family member 4 |
| 别名 | Ras association domain-containing protein 4, AD037 |
| 计算分子量 | 321 aa, 37 kDa |
| 观测分子量 | 34-37 kDa |
| GenBank蛋白编号 | BC032593 |
| 基因名称 | RASSF4 |
| Gene ID (NCBI) | 83937 |
| RRID | AB_10596921 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H2L5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RASSF4, a member of the classical RASSF family of scaffold proteins, is associated with alveolar rhabdomyosarcoma (aRMS), an aggressive pediatric cancer of muscle histogenesis. In aRMS, RASSF4 is upregulated in human cell lines and tumors and is oncogenic, portending a worse clinical prognosis. RASSF4 has been reported to directly interact with and activate K‐Ras in a GTP‐dependent manner. Ectopic expression of RASSF4 induces 293T apoptosis in a Ras‐dependent manner and inhibits tumour cell growth.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RASSF4 antibody 19118-1-AP | Download protocol |
| IHC protocol for RASSF4 antibody 19118-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Mol Med RASSF4 inhibits cell proliferation and increases drug sensitivity in colorectal cancer through YAP/Bcl-2 pathway.
| ||
Tumour Biol RASSF4 is downregulated in nonsmall cell lung cancer and inhibits cancer cell proliferation and invasion. |