验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26428-1-AP targets RASSF3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag15801 Product name: Recombinant human RASSF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 14-127 aa of BC100952 Sequence: KVQMELCKPPQTSPNSGKLSPSSNGCMNTLHISSTNTVGEVIEALLKKFLVTESPAKFALYKRCHREDQVYACKLSDREHPLYLRLVAGPRTDTLSFVLREHEIGEWEAFSLPE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Ras association (RalGDS/AF-6) domain family member 3 |
| 别名 | RASSF5 |
| 计算分子量 | 238 aa, 28 kDa |
| 观测分子量 | 28-30 kDa |
| GenBank蛋白编号 | BC100952 |
| 基因名称 | RASSF3 |
| Gene ID (NCBI) | 283349 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86WH2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RASSF3 (Ras Association Domain Family Member 3) is an emerging tumor suppressor protein and a key member of the RASSF family, characterized by a conserved Ras Association (RA) domain. RASSF3 acts as a critical node in multiple tumor-suppressive pathways. It directly stabilizes and activates the p53 protein, thereby inducing cell cycle arrest and apoptosis. RASSF3 also contributes to the maintenance of genomic integrity by participating in the DNA damage response. Furthermore, it modulates major signaling cascades such as the Hippo and NF-κB pathways, influencing cell proliferation, survival, and inflammation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RASSF3 antibody 26428-1-AP | Download protocol |
| WB protocol for RASSF3 antibody 26428-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
