验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue, rat lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 2 publications below |
产品信息
17971-1-AP targets RASIP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | RASIP1 fusion protein Ag11982 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Ras interacting protein 1 |
| 别名 | Ras-interacting protein 1, Ras interacting protein 1, Rain |
| 计算分子量 | 963 aa, 103 kDa |
| 观测分子量 | 115 kDa |
| GenBank蛋白编号 | BC028614 |
| 基因名称 | RASIP1 |
| Gene ID (NCBI) | 54922 |
| RRID | AB_2878474 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5U651 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RASIP1, also named as Ras-interacting protein 1 or Rain, is a 963 amino acid protein, which contains one dilute domain and one Ras-associating domian. RASIP1 is highly expressed in heart and detected at lower levels in placenta and pancreas. RASIP1 is required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. RASIP1 acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RASIP1 antibody 17971-1-AP | Download protocol |
| IHC protocol for RASIP1 antibody 17971-1-AP | Download protocol |
| IF protocol for RASIP1 antibody 17971-1-AP | Download protocol |
| IP protocol for RASIP1 antibody 17971-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Arterioscler Thromb Vasc Biol Adventitial Sca1+ Cells Transduced With ETV2 Are Committed to the Endothelial Fate and Improve Vascular Remodeling After Injury. | ||
Inflamm Regen A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo |
