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RASGRF2 Polyclonal antibody

RASGRF2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26788-1-AP

Print datasheet

Synonyms

GRF2, RAS GRF2, RASGRF2

验证数据展示

  • WB analysis using 26788-1-AP

    WB analysis using 26788-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26788-1-AP (RASGRF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 26788-1-AP

    IHC staining of human tonsillitis using 26788-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26788-1-AP (RASGRF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RASGRF2 antibody included

经过测试的应用

Positive WB detected inrat brain tissue, mouse brain tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RASGRF2 Ready-To-Use IHC Kit. RASGRF2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26788-1-AP targets RASGRF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen RASGRF2 fusion protein Ag25354 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Ras protein-specific guanine nucleotide-releasing factor 2
Synonyms GRF2, RAS GRF2, RASGRF2
Observed Molecular Weight141 kDa
GenBank Accession NumberBC136296
Gene Symbol RASGRF2
Gene ID (NCBI) 5924
RRIDAB_2918110
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14827
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RASGRF2 antibody 26788-1-APDownload protocol
IHC protocol for RASGRF2 antibody 26788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols