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验证数据展示

  • WB analysis using 26788-1-AP

    WB analysis using 26788-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26788-1-AP (RASGRF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 26788-1-AP

    IHC staining of human tonsillitis using 26788-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26788-1-AP (RASGRF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RASGRF2 antibody included

RASGRF2 Polyclonal antibody

RASGRF2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26788-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

GRF2, RAS GRF2, RASGRF2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue, mouse brain tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RASGRF2 Ready-To-Use IHC Kit. RASGRF2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26788-1-AP targets RASGRF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RASGRF2 fusion protein Ag25354 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Ras protein-specific guanine nucleotide-releasing factor 2
别名 GRF2, RAS GRF2, RASGRF2
观测分子量141 kDa
GenBank蛋白编号BC136296
基因名称 RASGRF2
Gene ID (NCBI) 5924
RRIDAB_2918110
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14827
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RASGRF2 antibody 26788-1-APDownload protocol
IHC protocol for RASGRF2 antibody 26788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols