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验证数据展示

  • WB analysis using 26788-1-AP

    WB analysis using 26788-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26788-1-AP (RASGRF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 26788-1-AP

    IHC staining of human tonsillitis using 26788-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26788-1-AP (RASGRF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RASGRF2 antibody included

RASGRF2 Polyclonal antibody

RASGRF2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26788-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

GRF2, RAS GRF2, RASGRF2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat brain tissue, mouse brain tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RASGRF2 Ready-To-Use IHC Kit. RASGRF2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

26788-1-AP targets RASGRF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag25354

Product name: Recombinant human RASGRF2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 748-876 aa of BC136296

Sequence: TSPLNSKIGALDLTTSSSPTTTTQSPAASPPPHTGQIPLDLSRGLSSPEQSPGTVEENVDNPRVDLCNKLKRSIQKAVLESAPADRAGVESSPAADTTELSPCRSPSTPRHLRYRQPGGQTADNAHCSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Ras protein-specific guanine nucleotide-releasing factor 2
别名 GRF2, RAS GRF2, RASGRF2
观测分子量141 kDa
GenBank蛋白编号BC136296
基因名称 RASGRF2
Gene ID (NCBI) 5924
RRIDAB_2918110
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14827
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for RASGRF2 antibody 26788-1-APDownload protocol
WB protocol for RASGRF2 antibody 26788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Discov Oncol

RASGRF2 as a potential pathogenic gene mediating the progression of alcoholic hepatitis to alcohol-related cirrhosis and hepatocellular carcinoma

Authors - Zhengyuan Chen
View Article
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